Quteish D, Dolby A E
Department of Periodontology, Dental School, University of Wales College of Medicine, Heath Park, Cardiff, U.K.
J Periodontal Res. 1991 Mar;26(2):114-21. doi: 10.1111/j.1600-0765.1991.tb01634.x.
Immunity to collagen implants may be mediated by cellular and humoral immune responses. To examine the possibility of such immunological reactivity and crossreactivity to collagen, 39 Sprague-Dawley rats (female, 10 weeks old, approximately 250 g wt) were implanted subcutaneously at thigh sites with crosslinked, freeze-dried human placental type I collagen grafts (4 x 4 x 2 mm) which had been irradiated (520 Gray) or left untreated. Blood was obtained by intracardiac sampling prior to implantation or from normal rats, and at various times afterwards when the animals were sacrificed. The sera from these animals were examined for circulating antibodies to human, bovine and rat tail (type I) collagens by enzyme-linked immunosorbent assay (ELISA). Also, the lymphoblastogenic responses of spleen lymphocytes from the irradiated collagen-implanted animals were assessed in culture by measuring thymidine uptake with autologous and normal rat sera in the presence of human and bovine type I collagens. Implantation of the irradiated and non-irradiated collagen grafts in rats led to a significant increase in the level of circulating antibodies to human collagen. Also antibody to bovine and rat tail collagens was detectable in the animals implanted with irradiated collagen grafts but at a lower level than the human collagen. There was a raised lymphoblastogenic response to both human and bovine collagens. The antibody level and lymphoblastogenesis to the tested collagens gradually decreased towards the end of the post-implantation period.
对胶原蛋白植入物的免疫可能由细胞免疫和体液免疫反应介导。为了研究对胶原蛋白产生这种免疫反应性和交叉反应性的可能性，将39只Sprague-Dawley大鼠（雌性，10周龄，体重约250克）在大腿部位皮下植入经交联、冻干的人胎盘I型胶原蛋白移植物（4×4×2毫米），这些移植物已接受辐照（520格雷）或未处理。在植入前或从正常大鼠获取血液，并在之后不同时间处死动物时获取血液。通过酶联免疫吸附测定（ELISA）检测这些动物血清中针对人、牛和大鼠尾巴（I型）胶原蛋白的循环抗体。此外，通过在人I型和牛I型胶原蛋白存在的情况下，用自体和正常大鼠血清测量胸腺嘧啶摄取，在培养中评估来自接受辐照的胶原蛋白植入动物的脾淋巴细胞的成淋巴细胞反应。在大鼠中植入辐照和未辐照的胶原蛋白移植物导致针对人胶原蛋白的循环抗体水平显著增加。在植入辐照胶原蛋白移植物的动物中也可检测到针对牛和大鼠尾巴胶原蛋白的抗体，但水平低于人胶原蛋白。对人胶原蛋白和牛胶原蛋白均有升高的成淋巴细胞反应。在植入后期，针对所测试胶原蛋白的抗体水平和成淋巴细胞反应逐渐降低。
