Imrey P B, Crawford J M, Cohen R L, Alves M E, McSwiggin T A, Chambers D A
Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois, Chicago.
J Periodontal Res. 1991 Mar;26(2):75-84. doi: 10.1111/j.1600-0765.1991.tb01629.x.
Previous investigation has shown that the concentration of aspartate aminotransferase (AST), an established serum marker for cardiac and liver damage in humans, is significantly elevated in samples of gingival crevicular fluid (GCF) from ligated teeth in beagle dogs. This paper reports on a cross-sectional study of the relationships between AST in GCF and clinical indices of human periodontal disease in 60 patients with mild to moderate adult periodontitis. AST standardized to a 30-second collection interval (AST30) showed substantial (multiple regression R2 = 0.61) association with summary indices of patient disease status, modest association (partial R2 = 0.22) with tooth disease status within patient, and weaker (partial R2 = 0.12) but statistically significant association with site-to-site variation in disease at the same tooth. AST concentration showed modest (R2 = 0.30) between-patient relationship with clinical indices, but no clinically significant relationship with these indices between sites within patients, suggesting a rough proportionality between accumulated enzyme and GCF volume at sites with varying stages of disease. The relationship between GCF volume and probing depth also appears central to interpretation of enzyme assays. Clinical measures of past periodontitis and current inflammatory disease are cross-sectionally related to variation in AST30, across patients and sites within the same patient. Considerable residual variation, especially elevated AST30 in the absence of clear signs of disease, may result from varying levels of current disease activity, not reflected in clinical measures.
先前的研究表明，天冬氨酸转氨酶（AST）作为人类心脏和肝脏损伤的一种既定血清标志物，在比格犬结扎牙齿的龈沟液（GCF）样本中浓度显著升高。本文报道了一项横断面研究，该研究针对60例轻至中度成人牙周炎患者，探讨了GCF中的AST与人类牙周病临床指标之间的关系。标准化为30秒采集间隔的AST（AST30）与患者疾病状态的综合指标显示出显著相关性（多元回归R2 = 0.61），与患者体内牙齿疾病状态存在适度相关性（偏R2 = 0.22），与同一颗牙齿不同部位间疾病差异的相关性较弱（偏R2 = 0.12），但具有统计学意义。AST浓度与临床指标在患者间的关系适度（R2 = 0.30），但在患者体内不同部位之间与这些指标无临床显著关系，这表明在疾病不同阶段的部位，累积酶与GCF体积之间大致成比例。GCF体积与探诊深度之间的关系似乎也是酶检测结果解读的核心。既往牙周炎和当前炎症性疾病的临床指标在横断面研究中与AST30的变化相关，涉及不同患者及同一患者的不同部位。相当大的残余变异，尤其是在无明显疾病迹象时AST30升高，可能是由于当前疾病活动水平不同所致，而这并未在临床指标中体现出来。
