Chambers D A, Imrey P B, Cohen R L, Crawford J M, Alves M E, McSwiggin T A
Center for Research in Periodontal Diseases and Oral Molecular Biology, University of Illinois, Chicago.
J Periodontal Res. 1991 Mar;26(2):65-74. doi: 10.1111/j.1600-0765.1991.tb01628.x.
Previous studies have shown that aspartate aminotransferase (AST), an established serum marker for cardiac and liver damage in humans, appears in elevated concentrations in samples of gingival crevicular fluid (GCF) from ligated vs. non-ligated teeth in beagle dogs and in elevated quantities in cross-sectional GCF sampling, adjusted for collection time, from human sites with clinical signs of past or present periodontal disease as compared to healthy sites. This paper describes a longitudinal study in which AST was monitored quarterly over a 2-year period at 2 sites/tooth in 31 patients with mild to moderate adult periodontitis. In this study sample, 40 (2.6%) of 1536 sites exhibited confirmed loss of at least 2 mm of attachment during the 2-yr observation period. In comparison with healthy sites within the same patients, AST standardized to a 30-second collection interval (AST30) was elevated at these sites with new confirmed attachment loss, and at sites with past attachment loss or gingivitis in the absence of periodontitis. When both within- and between-patient variation were taken into account, observed odds-ratios associating enzyme with disease were higher for sites with new attachment loss (9-16 depending on test cut-point) than for sites with pre-study attachment loss (3-12), or gingivitis in the absence of periodontitis (5-8). AST in GCF is strongly related to human periodontal disease. The data are consistent with the hypothesis that the relationship is strongest during episodes of cumulative tissue breakdown, but the small numbers of sites with confirmed attachment loss during the study period, or with gingivitis in the absence of periodontitis, means that further clinical studies are necessary to clarify this issue.
以往研究表明，天冬氨酸转氨酶（AST）作为人类心脏和肝脏损伤的一种既定血清标志物，在比格犬结扎牙与未结扎牙的龈沟液（GCF）样本中浓度升高，并且在根据采集时间进行调整后，与健康部位相比，在有过去或当前牙周疾病临床症状的人类部位的横断面GCF采样中含量升高。本文描述了一项纵向研究，在31例轻度至中度成人牙周炎患者中，对2个部位/颗牙的AST进行了为期2年的季度监测。在该研究样本中，1536个部位中有40个（2.6%）在2年观察期内出现至少2 mm附着丧失的确诊病例。与同一患者的健康部位相比，标准化为30秒采集间隔的AST（AST30）在这些有新确诊附着丧失的部位以及有过去附着丧失或无牙周炎的牙龈炎部位升高。当考虑患者内和患者间变异时，与疾病相关的酶的观察比值比在有新附着丧失的部位（根据检测切点为9 - 16）高于研究前有附着丧失的部位（3 - 12）或无牙周炎的牙龈炎部位（5 - 8）。GCF中的AST与人类牙周疾病密切相关。数据与以下假设一致，即这种关系在累积组织破坏发作期间最强，但研究期间有确诊附着丧失的部位数量较少，或有无牙周炎的牙龈炎部位数量较少，这意味着需要进一步的临床研究来阐明这个问题。
