Kim Tae Hoon, Barrera Leah O, Ren Bing
Yale University School of Medicine, New Haven, Connecticut, USA.
Curr Protoc Mol Biol. 2007 Jul;Chapter 21:Unit 21.13. doi: 10.1002/0471142727.mb2113s79.
ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. UNIT 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells.
染色质免疫沉淀芯片技术（ChIP-chip）将染色质免疫沉淀（ChIP）与微阵列（芯片）相结合，以确定活细胞中发生的蛋白质 - DNA相互作用。该方法的高通量特性使其成为识别全基因组转录因子靶点或染色质修饰位点的理想方法。第21.9单元描述了一种分析酵母细胞中蛋白质 - DNA相互作用的方案。本单元介绍了一种为哺乳动物细胞开发的替代方案。
