Hsu Wei-Cherng, Ritch Robert, Krupin Theodore, Chen Henry Shen-Lih
Department of Ophthalmology, Buddhist Tzu Chi General Hospital Taipei Branch, Taipei, Taiwan, Republic of China.
Graefes Arch Clin Exp Ophthalmol. 2008 May;246(5):709-17. doi: 10.1007/s00417-007-0744-9. Epub 2008 Feb 12.
To assess the effectiveness of OloGen (also named iGen), a porous, bioengineered, biodegradable, collagen-glycoaminoglycan matrix implant, in preventing poor bleb formation and early failure after trabeculectomy in eyes with a surgical wound defect.
The right eyes of 30 female New Zealand albino rabbits underwent trabeculectomy with OloGen implanted subconjunctivally on top of the scleral flap, while six right eyes received trabeculectomy without the implant to serve as a control group. A 1-2 mm diameter circular conjunctival defect was created in all eyes. Six rabbits in the group receiving the implant were sacrificed on days 3, 5, 7, 21, and 28. Rabbits in the control group were sacrificed on day 28. Perkins applanation tonometry, Seidel test and measurement of both the extent of the conjunctival defect and the anterior chamber depth were performed. Enucleated eyes were fixed in 4% formaldehyde and stained with hematoxylin and eosin (H&E) for general histological observation, and with Sirius and Fast-green stains to assess collagen deposition and cell migration.
Seidel tests were negative for all operated and control eyes. No flat anterior chamber occurred in either group. With the exception of days 5 and 7, post-operative mean IOP difference is significant in both groups, (P>0.05 for day 5, 7 and P<0.05 for day 3, 14, 21 and 28). In the implant group, the mean IOP was reduced by between 42% and 35% at days 14, 21, and 28, whereas the mean IOP in the control group was reduced by between only 12% and 2%. In the implant group, histology showed randomized collagen deposition and microcyst formation in the bleb after the matrix had degraded completely at day 28. In the control group, histology showed dense collagen deposition subconjunctivally at day 28.
OloGen successfully serves as a 3-dimensional scaffold for cell migration and proliferation, and can prevent failure by maintaining the size of the bleb in the presence of a large wound defect. It might also be successful at repairing postoperative bleb leaks.
评估OloGen（也称为iGen），一种多孔、生物工程、可生物降解的胶原-糖胺聚糖基质植入物，在预防手术伤口缺损的眼中小梁切除术后形成不良的滤过泡和早期失败方面的有效性。
30只雌性新西兰白化兔的右眼接受小梁切除术，并在巩膜瓣上方结膜下植入OloGen，而6只右眼接受小梁切除术但不植入作为对照组。所有眼睛均制造一个直径1-2毫米的圆形结膜缺损。接受植入物的组中的6只兔子在第3、5、7、21和28天处死。对照组的兔子在第28天处死。进行Perkins压平眼压测量、Seidel试验以及测量结膜缺损范围和前房深度。摘除的眼球固定在4%甲醛中，并用苏木精和伊红（H&E）染色进行一般组织学观察，并用天狼星和固绿染色评估胶原沉积和细胞迁移。
所有手术眼和对照眼的Seidel试验均为阴性。两组均未发生无前房。除第5天和第7天外，两组术后平均眼压差异均有统计学意义（第5、7天P>0.05，第3、14、21和28天P<0.05）。在植入物组中，第14、21和28天平均眼压降低了42%至35%，而对照组平均眼压仅降低了12%至2%。在植入物组中，组织学显示在第28天基质完全降解后滤过泡中有随机的胶原沉积和微囊肿形成。在对照组中，组织学显示在第28天结膜下有致密的胶原沉积。
OloGen成功地作为细胞迁移和增殖的三维支架，并能在存在大伤口缺损的情况下通过维持滤过泡大小来预防失败。它在修复术后滤过泡渗漏方面也可能成功。
Zotero 插件