Oh Y K, Freese E
J Bacteriol. 1976 Aug;127(2):739-46. doi: 10.1128/jb.127.2.739-746.1976.
In the absence of manganese, rapidly metabolizable carbohydrates such as glucose or glycerol are not completely metabolized by Bacillus subtilis growing in a nutrient sporulation medium: 3-phosphoglyceric acid (3PGA) accumulates inside the cells, growth stops at a low cell titer, and normal sporulation remains suppressed (no prespore septa). Upon the addition of manganese, 3PGA disappears, growth resumes, and normal sporulation takes place. These effects results from a specific manganese requirement of phosphoglycerate phosphomutase which catalyzes the interconversion of 3PGA and 2-phosphoglyceric acid (2PGA). Other metal ions cannot replace manganese, for which the enzyme has an apparent Km of 0.22 mM.
在缺乏锰的情况下，营养芽孢形成培养基中生长的枯草芽孢杆菌无法完全代谢葡萄糖或甘油等快速可代谢碳水化合物：3-磷酸甘油酸（3PGA）在细胞内积累，生长在低细胞密度时停止，正常芽孢形成仍受到抑制（无芽孢前体隔膜）。添加锰后，3PGA消失，生长恢复，正常芽孢形成发生。这些效应源于磷酸甘油酸磷酸变位酶对锰的特定需求，该酶催化3PGA和2-磷酸甘油酸（2PGA）的相互转化。其他金属离子无法替代锰，该酶对锰的表观米氏常数为0.22 mM。
