人类糖尿病视网膜病变中的小胶质细胞激活

Microglial activation in human diabetic retinopathy.

作者信息

Zeng Hui-yang, Green William Richard, Tso Mark O M

机构信息

Wilmer Eye Institute, Johns Hopkins Hospital, 600 N Wolfe St, Woods Bldg, Room 457, Baltimore, MD 21287-9238, USA.

出版信息

Arch Ophthalmol. 2008 Feb;126(2):227-32. doi: 10.1001/archophthalmol.2007.65.

DOI:10.1001/archophthalmol.2007.65

PMID:18268214

Abstract

OBJECTIVE

To investigate microglial activation in human diabetic retinopathy.

METHODS

Paraffin sections from 21 eyes of 13 patients with diabetic background, preproliferative, or proliferative retinopathies and 10 normal eyes of 9 individuals were studied with immunolabeling of microglia with antibodies against HLA-DR antigen, CD45, or CD68.

RESULTS

In the healthy human eyes, ramified microglial cells were scattered in the inner retinal layers. In eyes with diabetic retinopathy, the microglia were markedly increased in number and were hypertrophic at different stages of the disease. These cells clustered around the retinal vasculature, especially the dilated veins, microaneurysms, intraretinal hemorrhages, cotton-wool spots, optic nerve, and retinal and vitreal neovascularization. In some retinas with cystoid macular edema, microglia infiltrated the outer retina and subretinal space. Cells in the epiretinal membrane were also labeled with microglial markers.

CONCLUSIONS

Microglia were activated at different stages of human diabetic retinopathy and optic neuropathy. Microglial perivasculitis was a prominent feature of the disease process.

CLINICAL RELEVANCE

Activated microglia and microglial perivasculitis may play a role in vasculopathy and neuropathy in diabetic retinopathy.

摘要

目的

研究人类糖尿病视网膜病变中微胶质细胞的激活情况。

方法

对13例患有糖尿病背景、增殖前期或增殖性视网膜病变患者的21只眼的石蜡切片，以及9例个体的10只正常眼，用抗HLA - DR抗原、CD45或CD68抗体对微胶质细胞进行免疫标记研究。

结果

在健康人眼中，分支状微胶质细胞散在于视网膜内层。在糖尿病视网膜病变患者的眼中，微胶质细胞数量显著增加，且在疾病的不同阶段出现肥大。这些细胞聚集在视网膜血管周围，尤其是扩张的静脉、微动脉瘤、视网膜内出血、棉絮斑、视神经以及视网膜和玻璃体新生血管周围。在一些患有黄斑囊样水肿的视网膜中，微胶质细胞浸润到视网膜外层和视网膜下间隙。视网膜前膜中的细胞也被微胶质细胞标记物标记。