Tunon de Lara J M, Vuillemin L, Rondoyanni F, Rio P, Ducassou D, Taytard A
Department of Respiratory Diseases, Haut-Lévêque Hospital, CHU Bordeaux, France.
Clin Exp Allergy. 1991 Jan;21(1):109-13. doi: 10.1111/j.1365-2222.1991.tb00811.x.
The uptake of the monoamine radiolabelled tracer 125I-meta-iodo-benzyl-guanidine (MIBG) was studied in vitro in platelets from 15 healthy volunteers and five allergic patients whose serum IgE concentration was 37 +/- 33 and 650 +/- 200 IU/ml, respectively. The MIBG uptake was determined by means of a gamma counter after incubation in a buffer. Binding of IgE to platelets significantly reduced MIBG uptake both in healthy subjects and in allergic patients. This reduction was significantly related to the action of IgE as it did not occur after binding of IgG. Moreover, MIBG uptake plotted versus increasing concentration of IgE (ranging from 10 to 3000 IU/ml) was fitted by a classical sigmoidal curve which was not significantly different between healthy subjects and allergic patients. Lastly, the effect of IgE on MIBG uptake was due to the binding of the sole IgE as it was not modified by subsequent addition of anti-IgE. It is concluded that the binding of the sole IgE to its specific receptor on platelets alters the transport of monoamines.
在体外研究了15名健康志愿者和5名过敏患者血小板对单胺放射性标记示踪剂125I-间碘苄胍（MIBG）的摄取情况，这些过敏患者的血清IgE浓度分别为37±33和650±200 IU/ml。在缓冲液中孵育后，通过γ计数器测定MIBG摄取量。IgE与血小板的结合在健康受试者和过敏患者中均显著降低了MIBG摄取。这种降低与IgE的作用显著相关，因为在IgG结合后未出现这种情况。此外，将MIBG摄取量与递增的IgE浓度（范围为10至3000 IU/ml）作图，得到的经典S形曲线在健康受试者和过敏患者之间无显著差异。最后，IgE对MIBG摄取的影响是由于单一IgE的结合，因为随后添加抗IgE并未改变这种影响。结论是，单一IgE与其在血小板上的特异性受体结合会改变单胺的转运。
