Cardot E, Pestel J, Callebaut I, Lassalle P, Tsicopoulos A, Gras-Masse H, Capron A, Joseph M
Inserum CJF 90-06 URA CNRS 1309, Institut Pasteur, Lille, France.
Int Arch Allergy Immunol. 1992;98(2):127-34. doi: 10.1159/000236175.
Previous studies have shown that Fc epsilon RII-bearing platelets from patients with allergic disorders could be stimulated either by anti-human IgE antibodies or the relevant allergens to generate cytotoxic mediators. In this report, the reactivity of platelets from Dermatophagoides pteronyssinus (D. pt)-sensitive patients to three Der p I-derived synthetic peptides (52-71, 117-133 and 188-199) was investigated. The platelet stimulation observed in D. pt-sensitive patients was demonstrated to be allergen-specific and to be mediated by IgE. These Der p I-derived peptides failed to stimulate platelets from healthy donors or platelets from non-D. pt-allergic patients. D. pt-sensitive patients responded more frequently to the peptides 52-71 and 117-133 than to the peptide 188-199. Thus, in this model, synthetic peptides selected for their assumed accessibility on the native antigen could be associated to an IgE-dependent biological activity.
先前的研究表明，过敏性疾病患者携带FcεRII的血小板可被抗人IgE抗体或相关变应原刺激，从而产生细胞毒性介质。在本报告中，研究了对屋尘螨（D. pt）敏感患者的血小板对三种Der p I衍生合成肽（52-71、117-133和188-199）的反应性。在对D. pt敏感的患者中观察到的血小板刺激被证明是变应原特异性的，且由IgE介导。这些Der p I衍生肽未能刺激健康供体的血小板或非D. pt过敏患者的血小板。对D. pt敏感的患者对肽52-71和117-133的反应比对肽188-199更频繁。因此，在该模型中，因其在天然抗原上假定的可及性而选择的合成肽可能与IgE依赖性生物活性相关。
