Ridgway G L, Mumtaz G, Fenelon L
Department of Clinical Microbiology, University College Hospital, London, UK.
J Antimicrob Chemother. 1991 Feb;27 Suppl A:43-5. doi: 10.1093/jac/27.suppl_a.43.
The activity of oxytetracycline and seven macrolide antibiotics was investigated against a prototype strain of Chlamydia pneumoniae in cell culture. Following incubation, inoculated cell monolayers were fixed in methanol and stained with a genus specific immunofluorescent monoclonal antibody before examination for inhibition of inclusion formation. The most active agent was clarithromycin (MIC 0.007 mg/l), followed by erythromycin (0.06 mg/l). Other macrolides were less active; josamycin and roxithromycin (0.25 mg/l), midecamycin acetate and azithromycin (0.5 mg/l) and spiramycin (4.0 mg/l). The MIC of oxytetracycline was 0.25 mg/l. Clinical evaluation of clarithromycin for C. pneumoniae lower respiratory infection is indicated.
在细胞培养中研究了土霉素和七种大环内酯类抗生素对肺炎衣原体原型菌株的活性。孵育后，将接种的细胞单层用甲醇固定，并用属特异性免疫荧光单克隆抗体染色，然后检查包涵体形成的抑制情况。活性最强的药物是克拉霉素（MIC 0.007毫克/升），其次是红霉素（0.06毫克/升）。其他大环内酯类药物活性较低；交沙霉素和罗红霉素（0.25毫克/升）、醋酸麦迪霉素和阿奇霉素（0.5毫克/升）以及螺旋霉素（4.0毫克/升）。土霉素的MIC为0.25毫克/升。表明应对克拉霉素用于肺炎衣原体下呼吸道感染进行临床评估。
