Yoshida Fumiyo, Yamamoto Tetsuya, Nakai Kei, Kumada Hiroaki, Shibata Yasushi, Tsuruta Wataro, Endo Kiyoshi, Tsurubuchi Takao, Matsumura Akira
University of Tsukuba, Graduate School of Comprehensive Human Sciences, Functional and Regulatory Medical Sciences, Tennodai 1-1-1, Tsukuba, Ibaraki 305-8575, Japan.
Cancer Lett. 2008 May 18;263(2):253-8. doi: 10.1016/j.canlet.2008.01.016. Epub 2008 Feb 12.
We have previously reported that buthionine sulfoximine (BSO) enhances sodium borocaptate (BSH) uptake by down regulating glutathione (GSH) synthesis in cultured cells. This study investigated the influence of BSO on tissue BSH uptake in vivo and the efficacy of BSH-BSO-mediated boron neutron capture therapy (BNCT) on tumor growth using a Fisher-344 rat subcutaneous tumor model. With BSO supplementation, boron uptake in subcutaneous tumor, blood, skin, muscle, liver, and kidney was significantly enhanced and maintained for 12h. Tumor growth was significantly delayed by using BSO. With further improvement in experimental conditions, radiation exposure time, together with radiation damage to normal tissues, could be reduced.
我们之前报道过，丁硫氨酸亚砜胺（BSO）通过下调培养细胞中的谷胱甘肽（GSH）合成来增强硼酸钠盐（BSH）的摄取。本研究使用Fisher-344大鼠皮下肿瘤模型，研究了BSO对体内组织BSH摄取的影响以及BSH-BSO介导的硼中子俘获疗法（BNCT）对肿瘤生长的疗效。补充BSO后，皮下肿瘤、血液、皮肤、肌肉、肝脏和肾脏中的硼摄取显著增强，并维持12小时。使用BSO可显著延迟肿瘤生长。随着实验条件的进一步改善，辐射暴露时间以及对正常组织的辐射损伤都可以减少。
