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星形孢菌素既能激活又能抑制人血小板中的丝氨酸/苏氨酸激酶。

Staurosporine both activates and inhibits serine/threonine kinases in human platelets.

作者信息

Kocher M, Clemetson K J

机构信息

Theodor Kocher Institute, University of Berne, Switzerland.

出版信息

Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):301-6. doi: 10.1042/bj2750301.

DOI:10.1042/bj2750301
PMID:1827254
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1150052/
Abstract

The effects of staurosporine on a selection of protein kinases were investigated with thrombin-stimulated and control human blood platelets. The results demonstrate that staurosporine (1 microM) can lead to activation of certain protein kinases in intact platelets and has a general inhibitory effect on the renaturable protein kinases in vitro. New candidates for protein kinases involved in signal transduction are identified.

摘要

用凝血酶刺激的和未刺激的人血小板研究了星形孢菌素对一系列蛋白激酶的作用。结果表明，星形孢菌素（1微摩尔）可导致完整血小板中某些蛋白激酶的激活，并在体外对可复性蛋白激酶具有普遍抑制作用。确定了参与信号转导的蛋白激酶的新候选者。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/2bdf11c875b2/biochemj00161-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/f9c84fb2ca71/biochemj00161-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/eb37ba967113/biochemj00161-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/2bdf11c875b2/biochemj00161-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/f9c84fb2ca71/biochemj00161-0030-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/eb37ba967113/biochemj00161-0032-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8e16/1150052/2bdf11c875b2/biochemj00161-0033-a.jpg

相似文献

1
Staurosporine both activates and inhibits serine/threonine kinases in human platelets.星形孢菌素既能激活又能抑制人血小板中的丝氨酸/苏氨酸激酶。
Biochem J. 1991 Apr 15;275 ( Pt 2)(Pt 2):301-6. doi: 10.1042/bj2750301.
2
Serine/threonine kinases in signal transduction in response to thrombin in human platelets. Use of 17-hydroxywortmannin to discriminate signals.人血小板中响应凝血酶的信号转导中的丝氨酸/苏氨酸激酶。使用17-羟基渥曼青霉素区分信号。
Adv Exp Med Biol. 1993;344:119-28. doi: 10.1007/978-1-4615-2994-1_9.
3
Effects of 17-hydroxywortmannin on serine/threonine-protein kinases in human blood platelets.
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4
Activation of a serine/threonine kinase signaling pathway by transforming growth factor type beta.转化生长因子β激活丝氨酸/苏氨酸激酶信号通路。
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5
Phosphorylation of JAK2 in thrombin-stimulated human platelets.凝血酶刺激的人血小板中JAK2的磷酸化作用
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6
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7
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Thrombin-induced activation of the novel 33-kDa serine/threonine kinase in human platelets.凝血酶诱导人血小板中新型33 kDa丝氨酸/苏氨酸激酶的激活。
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Thrombin- and cathepsin G-induced platelet aggregation: effect of protein kinase C inhibitors.凝血酶和组织蛋白酶G诱导的血小板聚集：蛋白激酶C抑制剂的作用
Anal Biochem. 1993 Apr;210(1):50-7. doi: 10.1006/abio.1993.1149.

引用本文的文献

1
Inhibition of mitogen-activated protein kinase kinase does not impair primary activation of human platelets.丝裂原活化蛋白激酶激酶的抑制并不损害人血小板的初级激活。
Biochem J. 1996 Aug 15;318 ( Pt 1)(Pt 1):207-12. doi: 10.1042/bj3180207.
2
Inhibition of neuronal cyclin-dependent kinase-5 by staurosporine and purine analogs is independent of activation by Munc-18.
Neurochem Res. 1996 May;21(5):629-36. doi: 10.1007/BF02527763.
3
Activation of phospholipase C and protein kinase C has little involvement in ADP-induced primary aggregation of human platelets: effects of diacylglycerols, the diacylglycerols, the diacylglycerol kinase inhibitor R59022, staurosporine and okadaic acid.磷脂酶C和蛋白激酶C的激活在二磷酸腺苷诱导的人血小板初级聚集中几乎不起作用：二酰基甘油、二酰基甘油激酶抑制剂R59022、星形孢菌素和冈田酸的作用

本文引用的文献

1
Phorbol ester-induced activation of human platelets is associated with protein kinase C phosphorylation of myosin light chains.佛波酯诱导的人血小板激活与肌球蛋白轻链的蛋白激酶C磷酸化有关。
Nature. 1983;306(5942):490-2. doi: 10.1038/306490a0.
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Synergistic functions of protein phosphorylation and calcium mobilization in platelet activation.蛋白质磷酸化与钙动员在血小板激活中的协同作用。
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The role of protein kinase C in cell surface signal transduction and tumour promotion.蛋白激酶C在细胞表面信号转导及肿瘤促进中的作用。
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The role of protein kinase C and its neuronal substrates dephosphin, B-50, and MARCKS in neurotransmitter release.蛋白激酶C及其神经元底物去磷酸化蛋白、B-50和富含丙氨酸的豆蔻酰化蛋白激酶C底物在神经递质释放中的作用。
Mol Neurobiol. 1991;5(2-4):87-130. doi: 10.1007/BF02935541.
5
Regulation of phospholipase D by sphingosine involves both protein kinase C-dependent and -independent mechanisms in NIH 3T3 fibroblasts.在NIH 3T3成纤维细胞中，鞘氨醇对磷脂酶D的调节涉及蛋白激酶C依赖性和非依赖性机制。
Biochem J. 1992 Dec 15;288 ( Pt 3)(Pt 3):853-8. doi: 10.1042/bj2880853.
6
Activation of protein kinase C is not an absolute requirement for amylase release from permeabilized rat pancreatic acini.蛋白激酶C的激活并非通透化大鼠胰腺腺泡淀粉酶释放的绝对必要条件。
Biochem J. 1992 Jul 15;285 ( Pt 2)(Pt 2):597-601. doi: 10.1042/bj2850597.
7
Apical and basolateral Na/H exchange in cultured murine proximal tubule cells (MCT): effect of parathyroid hormone (PTH).培养的小鼠近端肾小管细胞（MCT）中顶端和基底外侧的钠/氢交换：甲状旁腺激素（PTH）的作用。
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4
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
5
A yeast gene that is essential for release from glucose repression encodes a protein kinase.一个对从葡萄糖阻遏中释放出来至关重要的酵母基因编码一种蛋白激酶。
Science. 1986 Sep 12;233(4769):1175-80. doi: 10.1126/science.3526554.
6
Staurosporine, a potent inhibitor of phospholipid/Ca++dependent protein kinase.星形孢菌素，一种磷脂/钙离子依赖性蛋白激酶的强效抑制剂。
Biochem Biophys Res Commun. 1986 Mar 13;135(2):397-402. doi: 10.1016/0006-291x(86)90008-2.
7
The protein kinase inhibitor staurosporine, like phorbol esters, induces the association of protein kinase C with membranes.蛋白激酶抑制剂星形孢菌素，与佛波酯一样，能诱导蛋白激酶C与细胞膜结合。
Biochem Biophys Res Commun. 1988 Aug 15;154(3):1273-9. doi: 10.1016/0006-291x(88)90277-x.
8
Monoclonal antibody recognition of two subtype forms of protein kinase C in human platelets.单克隆抗体对人血小板中蛋白激酶C两种亚型形式的识别。
Biochem Biophys Res Commun. 1988 Apr 29;152(2):642-8. doi: 10.1016/s0006-291x(88)80087-1.
9
The action of the protein kinase C inhibitor, staurosporine, on human platelets. Evidence against a regulatory role for protein kinase C in the formation of inositol trisphosphate by thrombin.蛋白激酶C抑制剂星形孢菌素对人血小板的作用。关于蛋白激酶C在凝血酶诱导肌醇三磷酸形成中起调节作用的证据不足。
Biochem J. 1988 Jan 15;249(2):345-50. doi: 10.1042/bj2490345.
10
Comparison of protein kinase C functional assays to clarify mechanisms of inhibitor action.比较蛋白激酶C功能测定以阐明抑制剂作用机制。
Biochem Pharmacol. 1988 Apr 15;37(8):1541-5. doi: 10.1016/0006-2952(88)90016-0.