Celenza J L, Carlson M
Science. 1986 Sep 12;233(4769):1175-80. doi: 10.1126/science.3526554.
The SNF1 gene plays a central role in carbon catabolite repression in the yeast Saccharomyces cerevisiae, namely that SNF1 function is required for expression of glucose-repressible genes. The nucleotide sequence of the cloned SNF1 gene was determined, and the predicted amino acid sequence shows that SNF1 encodes a 72,040-dalton polypeptide that has significant homology to the conserved catalytic domain of mammalian protein kinases. Specific antisera were prepared and used to identify the SNF1 protein. The protein was shown to transfer phosphate from adenosine triphosphate to serine and threonine residues in an in vitro autophosphorylation reaction. These findings indicate that SNF1 encodes a protein kinase and suggest that protein phosphorylation plays a critical role in regulation by carbon catabolite repression in eukaryotic cells.
SNF1基因在酿酒酵母的碳代谢物阻遏中起核心作用，即SNF1功能是葡萄糖可阻遏基因表达所必需的。已测定克隆的SNF1基因的核苷酸序列，预测的氨基酸序列表明SNF1编码一种72,040道尔顿的多肽，该多肽与哺乳动物蛋白激酶的保守催化结构域具有显著同源性。制备了特异性抗血清并用于鉴定SNF1蛋白。在体外自磷酸化反应中，该蛋白显示出将三磷酸腺苷中的磷酸转移至丝氨酸和苏氨酸残基。这些发现表明SNF1编码一种蛋白激酶，并提示蛋白磷酸化在真核细胞碳代谢物阻遏调控中起关键作用。
