Tyszkiewicz Amy B, Muir Tom W
Laboratory of Synthetic Protein Chemistry, The Rockefeller University, 1230 York Ave., New York, New York 10065, USA.
Nat Methods. 2008 Apr;5(4):303-5. doi: 10.1038/nmeth.1189. Epub 2008 Feb 13.
Spatiotemporal regulation of protein function is a key feature of living systems; experimental tools that provide such control are of great utility. Here we report a genetically encoded system for controlling a post-translational process, protein splicing, with light. Studies in Saccharomyces cerevisiae demonstrate that fusion of a photodimerization system from Arabidopsis thaliana to an artificially split intein permits rapid activation of protein splicing to yield a new protein product.
蛋白质功能的时空调节是生命系统的一个关键特征；能够提供这种控制的实验工具具有很大的实用价值。在此，我们报告了一种通过光来控制翻译后过程——蛋白质剪接的基因编码系统。在酿酒酵母中的研究表明，将来自拟南芥的光二聚化系统与人工拆分的内含肽融合，能够快速激活蛋白质剪接，从而产生一种新的蛋白质产物。
