Gerontology Institute of Shanxi Medical University, Taiyuan 030001, China
Neurosci Bull. 2008 Feb;24(1):13-20. doi: 10.1007/s12264-008-1017-2.
To explore effects of cerebral ischemia on the ceruloplasmin (Cp) expression in the cortex and hippocampus of rats.
Male Wistar rats were randomly divided into cerebral ischemia group and control group. Cerebral ischemia was induced by ligating bilateral common carotid arteries and the ischemic rats were further subgrouped according to ischemia time. The control rats received a sham operation. The expression of Cp mRNA in the cortex and hippocampus was measured by reverse transcription polymerase chain reaction (RT-PCR). The Cp expression was shown by immunohistochemistrical (streptavidin peroxidase, SP) method.
In ischemia group, the expression of Cp mRNA in the cortex and hippocampus decreased compared with that in control group (P< 0.01); and the longer rats experienced cerebral ischemia, the lower Cp mRNA expressed. By immunohistochemistry, Cp was shown expressed in the neural cells including epithelial cells of choroid plexus, ependymal cells, astrocytes of cortex and hippocampus, and vascular endothelial cells, but not in pyramidal cells and granulosa cells of cortex and hippocampus. Cp levels in the cortex and hippocampus decreased in rats suffering from cerebral ischemia for 3 d, 7 d and 28 d but not in rats exposed to ischemia for 1 d compared with that in control group (P< 0.05). Iron concentration correlated negatively with Cp expression in the cortex and hippocampus of rats exposure to ischemia (the cortex, r = -0.831, P< 0.01; the hippocampus, r = -0.809, P< 0.01).
Cerebral ischemia inhibited Cp expression in the cortex and hippocampus of rats. The decrease of Cp might be involved in iron deposition in neurons.
探讨脑缺血对大鼠皮质和海马脑铜蓝蛋白（Cp）表达的影响。
雄性 Wistar 大鼠随机分为脑缺血组和对照组。结扎双侧颈总动脉诱导脑缺血，再根据缺血时间将缺血大鼠进一步分为亚组。对照组大鼠接受假手术。采用逆转录聚合酶链反应（RT-PCR）检测皮质和海马 Cp mRNA 的表达。采用免疫组织化学（链霉亲和素过氧化物酶，SP）法显示 Cp 表达。
脑缺血组大鼠皮质和海马 Cp mRNA 的表达均低于对照组（P<0.01）；脑缺血时间越长，Cp mRNA 的表达越低。免疫组化显示 Cp 表达于脉络丛上皮细胞、室管膜细胞、皮质和海马星形胶质细胞及血管内皮细胞等神经细胞，而不表达于皮质和海马的锥体细胞和颗粒细胞。脑缺血 3、7 和 28 d 大鼠皮质和海马 Cp 水平较对照组降低（P<0.05），而脑缺血 1 d 大鼠与对照组比较差异无统计学意义。脑缺血大鼠皮质和海马铁浓度与 Cp 表达呈负相关（皮质，r=-0.831，P<0.01；海马，r=-0.809，P<0.01）。
脑缺血抑制了大鼠皮质和海马的 Cp 表达。Cp 的减少可能与神经元中铁沉积有关。
