Hattori Etsuko, Uchida Hiroshi, Harada Norihiro, Ohta Mari, Tsukada Hideo, Hara Yasuhiro, Suzuki Tetsuya
Bio Research Laboratory, Future Project Division, Toyota Motor Corporation, Toyota, Aichi, Japan.
Planta. 2008 Apr;227(5):1181-6. doi: 10.1007/s00425-008-0701-9. Epub 2008 Feb 14.
[(18)F]FDG (2-deoxy-2-[(18)F]fluoro-D-glucose) was fed to a sorghum plant [Sorghum bicolor (L.) Moench] from the tip of a leaf and its movement was monitored using a planar positron imaging system (PPIS). [(18)F]FDG was uptaken from the leaf tip and it was translocated to the basal part of the shoots from where it moved to the roots, the tillers and the sheaths. Autoradiographic analysis of the distribution of (18)F, [(18)F]FDG and/or its metabolites showed translocation to the roots, tillers, and to the leaves that were younger than the supplied leaf. Strong labelling was observed in the basal part of the shoots, in the sheaths, the youngest leaf and the root tips. Our results indicate that [(18)F]FDG and/or its metabolites were absorbed from the leaf and translocated to the sites where nutrients are required. This strongly suggests that [(18)F]FDG can be utilised as a tracer to study photoassimilate translocation in the living plant. This is the first report on the use of [(18)F]FDG, which is routinely used as a probe for clinical diagnosis, to study source to sink translocation of metabolites in whole plants in real time.
将[（18）F]FDG（2-脱氧-2-[（18）F]氟-D-葡萄糖）从叶片尖端施用于高粱植株[双色高粱（L.）Moench]，并使用平面正电子成像系统（PPIS）监测其移动情况。[（18）F]FDG从叶尖被吸收，并转运到茎基部，从那里它移动到根部、分蘖和叶鞘。对（18）F、[（18）F]FDG和/或其代谢产物分布的放射自显影分析表明，其转运到了根部、分蘖以及比施药叶片更年轻的叶片中。在茎基部、叶鞘、最幼嫩叶片和根尖观察到强烈的标记。我们的结果表明，[（18）F]FDG和/或其代谢产物从叶片中被吸收并转运到需要养分的部位。这有力地表明，[（18）F]FDG可作为一种示踪剂来研究活植物中光合产物的转运。这是关于使用[（18）F]FDG的首次报道，[（18）F]FDG通常用作临床诊断的探针，用于实时研究全株植物中代谢产物从源到库的转运。
