光激活水稻中的H2 15O流动：使用正电子发射示踪成像系统（PETIS）进行详细监测。
Light activates H2 15O flow in rice: Detailed monitoring using a positron-emitting tracer imaging system (PETIS).
作者信息
Kiyomiya S., Nakanishi H., Uchida H., Nishiyama S., Tsukada H., Ishioka N. S., Watanabe S., Osa A., Mizuniwa C., Ito T., Matsuhashi S., Hashimoto S., Sekine T., Tsuji A., Mori S.
机构信息
Depariment ofApplied Biological Chemistry, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan Takasaki Radiation Chemistry Research Establishment, Japan Atomic Energy Research Institute, Gunma 370-1207, Japan Central Research Laboratory Hamamatsu Photonics KK, Shizuoka 434-0047, Japan Present address: Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology Corporation (JST), 2-1-6 Sengen, Tsukuba 305-0047, Japan.
出版信息
Physiol Plant. 2001 Nov;113(3):359-367. doi: 10.1034/j.1399-3054.2001.1130309.x.
Water (H2 15O) translocation from the roots to the top of rice plants (Oryza saliva L. cv. Nipponbare) was visualized over time by a positron-emitting tracer imaging system (PETIS). H2 15O flow was activated 8 min after plants were exposed to bright light (1 500 &mgr;mol m-2 s-1). When the light was subsequently removed, the flow gradually slowed and completely stopped after 12 min. In plants exposed to low light (500 &mgr;mol m-2 s-1), H2 15O flow was activated more slowly, and a higher translocation rate of H2 15O was observed in the same low light at the end of the next dark period. NaCl (80 mM) and methylmercury (1 mM) directly suppressed absorption of H2 15O by the roots, while methionine sulfoximine (1 mM), abscisic acid (10 &mgr;M) and carbonyl cyanide m-chlorophenylhydrazone (10 mM) were transported to the leaves and enhanced stomatal closure, reducing H2 15O translocation.
利用正电子发射示踪成像系统（PETIS），对水稻（Oryza saliva L. cv. Nipponbare）植株中水分（H₂¹⁵O）从根部向顶部的转运随时间变化情况进行了可视化观察。在植株暴露于强光（1500 μmol m⁻² s⁻¹）8分钟后，H₂¹⁵O流动被激活。随后当光照去除后，水流逐渐减慢，并在12分钟后完全停止。在暴露于弱光（500 μmol m⁻² s⁻¹）的植株中，H₂¹⁵O流动激活得更慢，并且在下一个黑暗期结束时，在相同的弱光条件下观察到H₂¹⁵O有更高的转运速率。80 mM的NaCl和1 mM的甲基汞直接抑制根部对H₂¹⁵O的吸收，而1 mM的蛋氨酸亚砜亚胺、10 μM的脱落酸和10 mM的羰基氰化物间氯苯腙被转运到叶片并增强气孔关闭，从而减少H₂¹⁵O的转运。
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