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从本地分离的纤毛虫草履虫（Paramecium sp.）中首次鉴定并克隆出类镉金属硫蛋白基因

Identification and cloning of first cadmium metallothionein like gene from locally isolated ciliate, Paramecium sp.

作者信息

Shuja Rukhsana Nighat, Shakoori Abdul Rauf

机构信息

School of Biological Sciences, University of the Punjab, Lahore, Pakistan.

出版信息

Mol Biol Rep. 2009 Mar;36(3):549-60. doi: 10.1007/s11033-008-9213-8. Epub 2008 Feb 14.

DOI:10.1007/s11033-008-9213-8
PMID:18273691
Abstract

First cadmium metallothionein like gene PMCd1 of a ciliate, Paramecium sp., isolated from industrial wastewater has been cloned and sequenced. PMCd1 is an intronless gene, encoding 612 nucleotides, with TAA coding for glutamine. The coding region of PMCd1 comprises 203 amino acids, including 37 cysteine residues with a conserved structural pattern in the form of recurring structural motifs, arranged in 17 x-cys-x-y-cys-x, 1 x-cys-cys-x and x-cys-x contexts. Both, the deduced amino acids and nucleotide sequence differ, not only from other animal metallothioneins (MTs), but also from the previously characterized Tetrahymena Cu and Cd-MTs. The translated protein of PMCd1 contains conserved cysteine residues, peculiar characteristic of stress inducible metallothionein genes of ciliates and other groups of organisms.

摘要

从工业废水中分离出的一种纤毛虫——草履虫（Paramecium sp.）的首个类镉金属硫蛋白基因PMCd1已被克隆和测序。PMCd1是一个无内含子基因，编码612个核苷酸，其中TAA编码谷氨酰胺。PMCd1的编码区包含203个氨基酸，包括37个半胱氨酸残基，具有以重复结构基序形式存在的保守结构模式，排列为17个x-半胱氨酸-x-酪氨酸-半胱氨酸-x、1个x-半胱氨酸-半胱氨酸-x和x-半胱氨酸-x序列。推导的氨基酸序列和核苷酸序列不仅与其他动物金属硫蛋白（MTs）不同，也与先前鉴定的嗜热四膜虫铜和镉金属硫蛋白不同。PMCd1的翻译蛋白含有保守的半胱氨酸残基，这是纤毛虫和其他生物群体中应激诱导金属硫蛋白基因的独特特征。

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本文引用的文献

1
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Mar Biotechnol (NY). 2005 May-Jun;7(3):236-44. doi: 10.1007/s10126-004-0135-8. Epub 2005 May 5.
2
Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation.两个软体动物金属硫蛋白基因的定量PCR分析揭示了差异表达和调控。
Gene. 2005 Jan 31;345(2):259-70. doi: 10.1016/j.gene.2004.11.031. Epub 2005 Jan 6.
3
Diversity of metallothioneins in the American oyster, Crassostrea virginica, revealed by transcriptomic and proteomic approaches.
The Tetrahymena metallothionein gene family: twenty-one new cDNAs, molecular characterization, phylogenetic study and comparative analysis of the gene expression under different abiotic stressors.
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BMC Genomics. 2016 May 10;17:346. doi: 10.1186/s12864-016-2658-6.
4
The Fungus Tremella mesenterica Encodes the Longest Metallothionein Currently Known: Gene, Protein and Metal Binding Characterization.真菌胶锈菌编码目前已知最长的金属硫蛋白：基因、蛋白质及金属结合特性研究
PLoS One. 2016 Feb 16;11(2):e0148651. doi: 10.1371/journal.pone.0148651. eCollection 2016.
5
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Mol Biol Rep. 2013 Feb;40(2):983-97. doi: 10.1007/s11033-012-2140-8. Epub 2012 Nov 3.
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5
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6
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Gene. 2001 Jul 25;273(1):123-30. doi: 10.1016/s0378-1119(01)00577-7.
7
Antioxidant role of metallothioneins: a comparative overview.金属硫蛋白的抗氧化作用：比较综述
Cell Mol Biol (Noisy-le-grand). 2000 Mar;46(2):407-17.
8
Isolation and characterization of a self-sufficient one-domain protein. (Cd)-metallothionein from Eisenia foetida.一种自给自足的单结构域蛋白（镉）-金属硫蛋白的分离与鉴定，来自赤子爱胜蚓。
Eur J Biochem. 2000 Jan;267(2):573-82. doi: 10.1046/j.1432-1327.2000.01035.x.
9
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Biochem Pharmacol. 2000 Jan 1;59(1):95-104. doi: 10.1016/s0006-2952(99)00301-9.
10
Cadmium metallothionein gene of Tetrahymena pyriformis.
Gene. 1999 Jun 24;234(1):51-9. doi: 10.1016/s0378-1119(99)00181-x.