Porombka Doris, Baumgärtner Wolfgang, Eickmann Markus, Herden Christiane
Department of Pathology, University of Veterinary Medicine Hannover, Bünteweg 17, 30559 Hannover, Germany.
Virus Genes. 2008 Apr;36(2):415-20. doi: 10.1007/s11262-008-0210-8. Epub 2008 Feb 14.
The neurotropic Borna disease virus (BDV) causes typically a persistent virus infection of the central nervous system. In order to investigate whether an adapted virus replication contributes to BDV persistence in vivo, a fast and reliable real-time RT-PCR assay was constructed to quantify the amounts of leader-containing (leBDV) as a marker for virus replication, genomic (vBDV) and nucleoprotein-(BDV-N +ssRNA)-specific RNA. Therefore, leBDV, vBDV and BDV-N +ssRNA values were determined in experimentally infected Lewis rats between 14 and 90 days post infection (dpi). Surprisingly low leBDV values were found compared to vBDV and the abundantly expressed BDV-N transcripts. vBDV multiplied only in the acute phase of infection followed by constant expression until 90 dpi. Ratios of vBDV to leBDV were 401:1 at 14 dpi and diminished to 209:1 at 90 dpi, indicating a regulated co-expression of replicative intermediates as a potential prerequisite for viral persistence.
嗜神经性博尔纳病病毒（BDV）通常会引起中枢神经系统的持续性病毒感染。为了研究适应性病毒复制是否有助于BDV在体内的持续存在，构建了一种快速可靠的实时逆转录聚合酶链反应（RT-PCR）检测方法，以定量含前导序列的（leBDV）作为病毒复制标志物、基因组（vBDV）以及核蛋白特异性（BDV-N +ssRNA）RNA的量。因此，在感染后14至90天（dpi）测定了实验感染的Lewis大鼠中的leBDV、vBDV和BDV-N +ssRNA值。与vBDV和大量表达的BDV-N转录本相比，发现leBDV值出奇地低。vBDV仅在感染急性期增殖，随后持续表达直至90 dpi。vBDV与leBDV的比率在14 dpi时为401:1，在90 dpi时降至209:1，表明复制中间体的调节共表达是病毒持续存在的潜在先决条件。
