Pyper J M, Clements J E, Zink M C
Division of Comparative Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
J Virol. 1998 Sep;72(9):7697-702. doi: 10.1128/JVI.72.9.7697-7702.1998.
Borna disease virus (BDV) is a neurotropic nonsegmented negative-strand RNA virus with limited homology to rhabdoviruses and paramyxoviruses. A distinguishing feature of BDV is that it replicates in the nucleus of infected cells. Strand-specific probes used for in situ hybridization of infected rat brain showed that there was differential localization of positive- and negative-strand RNAs within the nucleus of neurons. Within nuclei, sense-strand RNAs were preferentially localized within nucleolar regions while genomic-sense RNAs were found in both nucleolar and nonnucleolar regions. These results suggested a role for the nucleolus in BDV replication. Nucleoli isolated from persistently infected neuroblastoma cells contained both genomic and antigenomic BDV RNA species as well as an enrichment of the 39/38-kDa and gp18 BDV proteins. Since the nucleolus is the site of rRNA transcription, we examined BDV transcription in the presence of inhibitors of RNA polymerase I. Inhibition of RNA polymerase I did not affect levels of BDV transcription.
博尔纳病病毒（BDV）是一种嗜神经的不分节段负链RNA病毒，与弹状病毒和副粘病毒的同源性有限。BDV的一个显著特征是它在受感染细胞的细胞核中复制。用于感染大鼠脑原位杂交的链特异性探针显示，神经元细胞核内正链和负链RNA存在差异定位。在细胞核内，正义链RNA优先定位于核仁区域，而基因组正义RNA则在核仁区域和非核仁区域均有发现。这些结果提示核仁在BDV复制中发挥作用。从持续感染的神经母细胞瘤细胞中分离出的核仁含有基因组和反基因组BDV RNA种类，以及39/38-kDa和gp18 BDV蛋白的富集。由于核仁是rRNA转录的场所，我们在存在RNA聚合酶I抑制剂的情况下检测了BDV转录。RNA聚合酶I的抑制并不影响BDV转录水平。
