核糖体蛋白是NEDD8途径的作用靶点。
Ribosomal proteins are targets for the NEDD8 pathway.
作者信息
Xirodimas Dimitris P, Sundqvist Anders, Nakamura Akihiro, Shen Linnan, Botting Catherine, Hay Ronald T
机构信息
Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
出版信息
EMBO Rep. 2008 Mar;9(3):280-6. doi: 10.1038/embor.2008.10. Epub 2008 Feb 15.
Abstract
Identification of the molecular targets for post-translational modifications is an important step for explaining the regulated pathways. The ubiquitin-like molecule NEDD8 is implicated in the regulation of cell proliferation, viability and development. By combining proteomics and in vivo NEDDylation assays, we identified a subset of ribosomal proteins as novel targets for the NEDD8 pathway. We further show that the lack of NEDDylation in cells causes ribosomal protein instability. Our studies identify a novel and specific role of the NEDD8 pathway in protecting a subset of ribosomal proteins from destabilization.
摘要
鉴定翻译后修饰的分子靶点是解释调控途径的重要一步。类泛素分子NEDD8参与细胞增殖、活力和发育的调控。通过结合蛋白质组学和体内NEDDylation分析，我们鉴定出一组核糖体蛋白作为NEDD8途径的新靶点。我们进一步表明，细胞中NEDDylation的缺失会导致核糖体蛋白不稳定。我们的研究确定了NEDD8途径在保护一部分核糖体蛋白免于不稳定方面的新的特定作用。
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