Chen Gang, Shi Jinxin, Ding Yasuo, Yin Hongxia, Hang Chunhua
Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, Jiangsu Province 210002, China.
Mediators Inflamm. 2007;2007:93431. doi: 10.1155/2007/93431.
We have previously shown that traumatic brain injury (TBI) can induce an upregulation of nuclear factor kappa B (NF-kappaB) and proinflammatory cytokines in the gut, which play an important role in the pathogenesis of acute gut mucosal injury mediated by inflammation. In this work, we investigated whether progesterone administration modulated intestinal NF-kappaB activity and proinflammatory cytokines expression after TBI in male rats. As a result, we found that administration of progesterone following TBI could decrease NF-kappaB binding activity, NF-kappaB p65 protein expression, and concentrations of interleukin-1beta (IL-1beta), and tumor necrosis factor-alpha (TNF-alpha) in the gut. TBI-induced damages of gut structure were ameliorated after progesterone injections. The results of the present study suggest that the therapeutic benefit of post-TBI progesterone injections might be due to its inhibitory effects on intestinal NF-kappaB activation and proinflammatory cytokines expression.
我们之前已经表明，创伤性脑损伤（TBI）可诱导肠道中核因子κB（NF-κB）和促炎细胞因子上调，它们在炎症介导的急性肠黏膜损伤发病机制中起重要作用。在这项研究中，我们调查了给予孕酮是否能调节雄性大鼠TBI后肠道NF-κB活性和促炎细胞因子表达。结果发现，TBI后给予孕酮可降低肠道中NF-κB结合活性、NF-κB p65蛋白表达以及白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的浓度。孕酮注射后，TBI诱导的肠道结构损伤得到改善。本研究结果表明，TBI后注射孕酮的治疗益处可能归因于其对肠道NF-κB激活和促炎细胞因子表达的抑制作用。
