Meczynska Sylwia, Lewandowska Hanna, Sochanowicz Barbara, Sadlo Jaroslaw, Kruszewski Marcin
Department of Radiobiology and Health Protection, Institute of Nuclear Chemistry and Technology, Warsaw, Poland.
Hemoglobin. 2008;32(1-2):157-63. doi: 10.1080/03630260701699821.
A prerequisite of dinitrosyl iron complexes (DNIC) formation is the presence of nitric oxide (NO), iron (Fe) and thiol/imidazole groups. The aim of this study was to investigate the influence of Fe chelators on the formation of DNIC in erythroid K562 cells. The cells were treated with lipophilic salicylaldehyde isonicotinoyl hydrazone (SIH) (0.1 mM) and hydrophilic deferoxamine mesylate (DFO) (1 mM), a membrane permeable and non permeable Fe chelator, respectively. Dinitrosyl Fe complexes were generated by addition of 0.07 mM diethylamine NO. The DNIC formation was recorded using electron paramagnetic resonance (EPR). Both chelators inhibited DNIC formation up to 50% after 6 hours of treatment. Taken together, our data suggest that an intracellular low molecular weight labile Fe pool (LIP) and protein-bound Fe participate in DNIC formation in K562 cells to a similar extent.
二亚硝基铁配合物（DNIC）形成的一个先决条件是存在一氧化氮（NO）、铁（Fe）和硫醇/咪唑基团。本研究的目的是调查铁螯合剂对红系K562细胞中DNIC形成的影响。细胞分别用亲脂性的水杨醛异烟酰腙（SIH）（0.1 mM）和亲水性的甲磺酸去铁胺（DFO）（1 mM）处理，后者分别是一种可透过膜和不可透过膜的铁螯合剂。通过添加0.07 mM二乙胺NO来生成二亚硝基铁配合物。使用电子顺磁共振（EPR）记录DNIC的形成。两种螯合剂在处理6小时后均将DNIC的形成抑制了50%。综上所述，我们的数据表明，细胞内低分子量不稳定铁池（LIP）和蛋白质结合铁在K562细胞的DNIC形成中发挥了相似程度的作用。
