Yao Jih-Guang, Weasner Bonnie M, Wang Lan-Hsin, Jang Chuen-Chuen, Weasner Brandon, Tang Chiou-Yang, Salzer Claire L, Chen Chun-Hong, Hay Bruce, Sun Y Henry, Kumar Justin P
Institute of Molecular Biology, Academia Sinica, Taipei 11529, Taiwan, ROC.
Dev Biol. 2008 Mar 15;315(2):535-51. doi: 10.1016/j.ydbio.2007.12.037. Epub 2008 Jan 4.
In eye development the tasks of tissue specification and cell proliferation are regulated, in part, by the Pax6 and Pax6(5a) proteins respectively. In vertebrates, Pax6(5a) is generated as an alternately spliced isoform of Pax6. This stands in contrast to the fruit fly, Drosophila melanogaster, which has two Pax6(5a) homologs that are encoded by the eyegone and twin of eyegone genes. In this report we set out to determine the respective contributions that each gene makes to the development of the fly retina. Here we demonstrate that both eyg and toe encode transcriptional repressors, are expressed in identical patterns but at significantly different levels. We further show, through a molecular dissection of both proteins, that Eyg makes differential use of several domains when compared to Toe and that the number of repressor domains also differs between the two Pax6(5a) homologs. We predict that these results will have implications for elucidating the functional differences between closely related members of other Pax subclasses.
在眼睛发育过程中，组织特化和细胞增殖任务分别部分地受Pax6和Pax6(5a)蛋白调控。在脊椎动物中，Pax6(5a)是作为Pax6的一个选择性剪接异构体产生的。这与果蝇（黑腹果蝇）形成对比，果蝇有两个由eyegone和eyegone的孪生基因编码的Pax6(5a)同源物。在本报告中，我们着手确定每个基因对果蝇视网膜发育的各自贡献。在这里我们证明，eyg和toe都编码转录抑制因子，以相同模式表达但水平显著不同。我们通过对这两种蛋白质的分子剖析进一步表明，与Toe相比，Eyg对几个结构域的利用有所不同，并且这两个Pax6(5a)同源物之间的抑制结构域数量也不同。我们预测这些结果将对阐明其他Pax亚类密切相关成员之间的功能差异具有启示意义。
