利用光片显微镜进行 5D 成像揭示了果蝇眼-触角盘原基形成过程中的细胞动态。

5D imaging via light sheet microscopy reveals cell dynamics during the eye-antenna disc primordium formation in Drosophila.

机构信息

Institute of Biophotonics, National Yang-Ming University, Taipei, Taiwan.

Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan.

出版信息

Sci Rep. 2017 Mar 21;7:44945. doi: 10.1038/srep44945.

DOI:10.1038/srep44945

PMID:28322328

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5359570/

Abstract

5D images of engrailed (en) and eye gone (eyg) gene expressions during the course of the eye-antenna disc primordium (EADP) formation of Drosophila embryos from embryonic stages 13 through 16 were recorded via light sheet microscopy and analyzed to reveal the cell dynamics involved in the development of the EADP. Detailed analysis of the time-lapsed images revealed the process of EADP formation and its invagination trajectory, which involved an inversion of the EADP anterior-posterior axis relative to the body. Furthermore, analysis of the en-expression pattern in the EADP provided strong evidence that the EADP is derived from one of the en-expressing head segments.

摘要

通过光片显微镜记录了果蝇胚胎从 13 到 16 阶段期间， engrailed(en) 和 eye gone(eyg) 基因表达的 5D 图像，并对其进行了分析，以揭示参与眼-触角盘原基（EADP）形成的细胞动力学。对时移图像的详细分析揭示了 EADP 的形成过程及其内陷轨迹，这涉及到 EADP 相对于身体的前后轴的反转。此外，对 EADP 中 en 表达模式的分析提供了强有力的证据，表明 EADP 源自一个表达 en 的头部节段。