[培养基碳源对耻垢分枝杆菌抗结核药物敏感性的作用]

[Role of carbon substrates of culture media on Mycobacterium smegmatis susceptibility to antitubercular agents].

作者信息

Shakina Iu V, Troshkina O A, Petrova E E, Salina E G, Sorokoumova G M, Shvets V I, Kapel'iants A S, Selishcheva A A

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 2007 Nov-Dec(6):7-11.

Abstract

Influence of medium composition on Mycobacterium smegmatis growth and susceptibility to antituberculosis drugs (ATD)--isoniazid and rifabutin--was studied. It was shown that addition of phospholipids (PL) in form of liposomes to meat peptone broth resulted in activation of M. smegmatis growth and decrease of its susceptibility to ATD. Growth characteristics of M. smegmatis and its susceptibility to ATD were studied using variants of modified on source of carbon synthetic medium Sauton as growth substrate. It was revealed that presence of acetate or PL in growth medium results in significant decrease of M. smegmatis susceptibility to isoniazid and rifabutin. It was suggested that this phenomenon is determined by activation of glyoxylate cycle by PL and fatty acids, which, in its turn, can stimulate expression of a number of proteins, including cell membrane pumps excreting antibiotics out of microbial cell.

摘要

研究了培养基成分对耻垢分枝杆菌生长以及对异烟肼和利福布汀这两种抗结核药物（ATD）敏感性的影响。结果表明，向肉蛋白胨肉汤中添加脂质体形式的磷脂（PL）会导致耻垢分枝杆菌生长活化，并降低其对ATD的敏感性。以改良的基于碳源的合成培养基Sauton变体作为生长底物，研究了耻垢分枝杆菌的生长特性及其对ATD的敏感性。结果发现，生长培养基中存在乙酸盐或PL会导致耻垢分枝杆菌对异烟肼和利福布汀的敏感性显著降低。有人认为，这一现象是由PL和脂肪酸激活乙醛酸循环所决定的，而乙醛酸循环反过来又会刺激包括将抗生素排出微生物细胞的细胞膜泵在内的多种蛋白质的表达。