Troshkina O A, Salina E G, Sorokoumova G M, Kaprel'iants A S, Selishcheva A A
Prikl Biokhim Mikrobiol. 2007 Jan-Feb;43(1):47-52.
The effects of the liposome form of isoniazide (IN) and liposomes without IN on the growth of Mycobacterium smegmatis were studied. Fluorescent assay demonstrated that the fraction of liposomes that interacted with M. smegmatis amounted to 1-3%. It was shown that the IN efficiency in a liposomal form decreased depending on the liposome composition and concentration as compared with the IN in water solution. A preincubation of mycobacteria with liposomes led to a decrease in their sensitivity to IN. An analogous effect was observed when incubating M. smegmatis with oleic acid. It was postulated that the relative resistance of M. smegmatis to the antibiotic when using lipids as a carbon substrate appeared due to a change in the agent's metabolism and should be taken into account when testing in vitro the liposomal forms of antibiotics.
研究了异烟肼（IN）脂质体形式以及不含IN的脂质体对耻垢分枝杆菌生长的影响。荧光分析表明，与耻垢分枝杆菌相互作用的脂质体比例为1%-3%。结果显示，与水溶液中的IN相比，脂质体形式的IN效率会根据脂质体的组成和浓度而降低。分枝杆菌与脂质体预孵育会导致其对IN的敏感性降低。用油酸孵育耻垢分枝杆菌时也观察到类似效果。据推测，当使用脂质作为碳源时，耻垢分枝杆菌对抗生素的相对抗性是由于药物代谢的改变而出现的，在体外测试抗生素脂质体形式时应予以考虑。
