Brown Leif O, Doorn Stephen K
Chemical Sciences and Engineering, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.
Langmuir. 2008 Mar 18;24(6):2277-80. doi: 10.1021/la703853e. Epub 2008 Feb 16.
Silver nanoparticles tagged with dyes and encapsulated within a silica layer, offer a convenient potential substrate for performing multiplexed surface-enhanced Raman scattering (SERS) analysis. In contrast to our earlier work with gold particles, aggregation of silver particles is found to be mostly independent of dye addition, allowing for a reproducible preparation in which aggregation is actively induced by the addition of NaCl. Separating the aggregation step eliminates competitive binding between the dyes and silica-coating reagents, enabling the efficient use of a wide variety of weakly binding dyes to conveniently generate robust, high-intensity SERS substrates at a variety of excitation frequencies.
标记有染料并封装在二氧化硅层内的银纳米颗粒，为进行多重表面增强拉曼散射（SERS）分析提供了一种方便的潜在底物。与我们早期使用金颗粒的工作相比，发现银颗粒的聚集大多与染料添加无关，这使得通过添加氯化钠来主动诱导聚集的可重复制备成为可能。分离聚集步骤消除了染料与二氧化硅涂层试剂之间的竞争性结合，从而能够有效利用各种弱结合染料，在各种激发频率下方便地生成稳定、高强度的SERS底物。
