Di Pasqua Anthony J, Sharma Krishna K, Shi Yan-Li, Toms Bonnie B, Ouellette Wayne, Dabrowiak James C, Asefa Tewodros
Department of Chemistry, Syracuse University, 111 College Place, Syracuse, NY 13244-4100, USA.
J Inorg Biochem. 2008 Jul;102(7):1416-23. doi: 10.1016/j.jinorgbio.2007.12.028. Epub 2008 Jan 9.
We here measure the toxicity of MCM-41, a mesoporous silica nanomaterial, two of its functionalized analogs, AP-T, which has grafted aminopropyl groups and MP-T, which has grafted mercaptopropyl groups, and spherical silica nanoparticles (SiO(2)), toward human neuroblastoma (SK-N-SH) cells. Since the particles studied are not soluble in aqueous media, the metric used to report the cytotoxicity of these materials is a new quantity, Q(50), which is the number of particles required to inhibit normal cell growth by 50%. Determining the number of particles per gram of material applied to the cells required both the calculated and experimentally determined surface areas of these nanomaterials. This study shows that Q(50) increases in the order, MCM-41<MP-T<AP-T approximately SiO(2), showing that on a per particle basis, MCM-41 is the most cytotoxic material studied. For the three mesoporous silica materials in this study, cytotoxicity appears related to the adsorptive surface area of the particle, although the nature of the functional group cannot be ruled out. Silica nanospheres have the lowest surface area of the particles studied but since they exhibit a Q(50) value similar to that of AP-T, shape may also be important in the cytotoxicity of these materials.
我们在此测定介孔二氧化硅纳米材料MCM - 41、其两种功能化类似物（接枝氨丙基的AP - T和接枝巯基丙基的MP - T）以及球形二氧化硅纳米颗粒（SiO₂）对人神经母细胞瘤（SK - N - SH）细胞的毒性。由于所研究的颗粒不溶于水性介质，用于报告这些材料细胞毒性的指标是一个新的量Q(50)，即抑制正常细胞生长50%所需的颗粒数量。确定施加到细胞上的每克材料的颗粒数量既需要计算这些纳米材料的表面积，也需要通过实验测定其表面积。本研究表明，Q(50)按MCM - 41＜MP - T＜AP - T≈SiO₂的顺序增加，这表明就单个颗粒而言，MCM - 41是所研究的细胞毒性最大的材料。对于本研究中的三种介孔二氧化硅材料，细胞毒性似乎与颗粒的吸附表面积有关，尽管不能排除官能团性质的影响。在所研究的颗粒中，二氧化硅纳米球的表面积最小，但由于它们的Q(50)值与AP - T相似，形状可能在这些材料的细胞毒性中也很重要。
