Pierrez J, Ronot X
Eurocyt, Strasbourg, France.
Cytometry. 1991;12(3):275-8. doi: 10.1002/cyto.990120311.
DNA content determination requires the use of standards. Vindelov has shown the need to use two standards. Chicken and trout erythrocytes are commonly used, but they are not ideal standards. On the one hand, their DNA contents rarely frame the studied sample DNA content, and, on the other hand, as their base compositions are different in terms of A + T/G + C, their relative indices change according to the stains used. Use of triploid trout erythrocytes instead of chicken erythrocytes allows elimination of these two drawbacks; however, diploid trout must be differentiated from triploid trout. The present paper shows that an anatomic malformation is found with the triploid trout and so justifies the use of paired diploid and triploid trout as standards to measure nuclear DNA content.
DNA含量的测定需要使用标准品。温德洛夫已经表明需要使用两种标准品。鸡和鳟鱼的红细胞是常用的，但它们并非理想的标准品。一方面，它们的DNA含量很少能涵盖所研究样本的DNA含量范围；另一方面，由于它们在A+T/G+C方面的碱基组成不同，其相对指数会根据所使用的染色剂而变化。使用三倍体鳟鱼的红细胞而非鸡的红细胞可以消除这两个缺点；然而，必须将二倍体鳟鱼与三倍体鳟鱼区分开来。本文表明，三倍体鳟鱼存在解剖学畸形，因此证明使用配对的二倍体和三倍体鳟鱼作为标准品来测量核DNA含量是合理的。
