Espinosa Emilio, Josa Agustín, Gil Lidia, Martí José Ignacio
Reproducción Ictiológica (Departamento de Patología Animal), Facultad de Veterinaria, Miguel Servet 177, 50013 Zaragoza, Spain.
J Exp Zool A Comp Exp Biol. 2005 Nov 1;303(11):1007-12. doi: 10.1002/jez.a.223.
This study was designed to assess the use of a computer-assisted system based on erythrocyte measurements as a possible alternative to flow cytometry for identifying triploid rainbow trout (Oncorhynchus mykiss). Blood smears were prepared from 26 triploid and 26 diploid specimens, as determined by flow cytometry after staining blood cells with propidium iodide. The cell and nucleus lengths of 10 erythrocytes were determined in each fish. This was followed by discriminatory analysis to distinguish between diploids and triploids based on their score profiles. Triploid trout showed significantly larger erythrocyte cell and nucleus measurements than their diploid counterparts (N=52; P<0.0001). Erythrocyte length correctly identified 100% of the fish specimens as diploid or triploid, while nucleus length was a less accurate predictor of the level of ploidy. Our findings validate the potential use of computer-assisted analysis for this purpose.
本研究旨在评估基于红细胞测量的计算机辅助系统作为鉴定三倍体虹鳟（Oncorhynchus mykiss）的一种可能替代流式细胞术的方法。用碘化丙啶对血细胞进行染色后，通过流式细胞术确定了26个三倍体和26个二倍体样本，并制备了血涂片。在每条鱼中测定10个红细胞的细胞和细胞核长度。随后进行判别分析，根据二倍体和三倍体的得分概况对它们进行区分。三倍体鳟鱼的红细胞细胞和细胞核测量值明显大于其二倍体对应物（N = 52；P < 0.0001）。红细胞长度能正确地将100%的鱼样本鉴定为二倍体或三倍体，而细胞核长度作为倍性水平的预测指标则不太准确。我们的研究结果验证了为此目的使用计算机辅助分析的潜力。
