Rogers Pamela M, Ying Ling, Burris Thomas P
Nuclear Receptor Biology Laboratory, Pennington Biomedical Research Center, Louisiana State University System, 6400 Perkins Road, LA 70808, USA.
Biochem Biophys Res Commun. 2008 Apr 18;368(4):955-8. doi: 10.1016/j.bbrc.2008.02.031. Epub 2008 Feb 15.
The nuclear hormone receptors, REV-ERBalpha [NR1D1] and REV-ERBbeta [NR1D1], were recently demonstrated to be receptors for the porphyrin, heme. Heme regulates the ability of these receptors to repress transcription of their target genes via modulation of the affinity of the receptor's ligand binding domain for the corepressor, NCoR. The REV-ERBs function as critical components of the mammalian clock and their expression oscillates in a circadian manner. Here, we show that in NIH3T3 cells intracellular heme levels also oscillate in a circadian fashion. These data are the first to show the temporal relationship of intracellular heme levels to the expression of its receptor, Rev-erbalpha, and suggest that the rapid oscillations in heme levels may an important component regulating REV-ERB transcriptional activity.
核激素受体REV-ERBα[NR1D1]和REV-ERBβ[NR1D1]最近被证明是卟啉血红素的受体。血红素通过调节受体配体结合域与共抑制因子NCoR的亲和力，来调节这些受体抑制其靶基因转录的能力。REV-ERB作为哺乳动物生物钟的关键组成部分，其表达以昼夜节律的方式振荡。在此，我们表明在NIH3T3细胞中，细胞内血红素水平也以昼夜节律的方式振荡。这些数据首次显示了细胞内血红素水平与其受体Rev-erbalpha表达的时间关系，并表明血红素水平的快速振荡可能是调节REV-ERB转录活性的一个重要组成部分。
