Gadhia P, Gadhia M, Zankl H
Department of Biosciences, South Gujarat University, Surat, India.
Mutagenesis. 1991 Jan;6(1):31-3. doi: 10.1093/mutage/6.1.31.
Blood samples from six Down's syndrome (DS) and six age- and sex-matched controls were cultured for 72 h in the presence of BrdUrd. Lymphocytes were then analysed at their second mitosis for sister chromatid exchange (SCE) and at their first mitosis for chromosome aberrations. Treatment with adriamycin (30 and 60 ng) showed a significant increase in frequency of SCE and chromosome aberrations in DS lymphocytes compared to normal lymphocytes at initiation of culture. Cells treated with adriamycin (ADR) for the last 24 h also showed a significant increase in SCE in DS lymphocytes compared to normal lymphocytes. A significant increase in chromatid-type aberrations was also recorded in DS lymphocytes after both treatments cultured for the last 24 h.
取6名唐氏综合征（DS）患者以及6名年龄和性别匹配的对照者的血样，在存在溴脱氧尿苷（BrdUrd）的情况下培养72小时。然后在淋巴细胞的第二次有丝分裂时分析姐妹染色单体交换（SCE），并在第一次有丝分裂时分析染色体畸变。与培养开始时的正常淋巴细胞相比，用阿霉素（30和60纳克）处理后，DS淋巴细胞的SCE频率和染色体畸变显著增加。在培养的最后24小时用阿霉素（ADR）处理的细胞，与正常淋巴细胞相比，DS淋巴细胞的SCE也显著增加。在培养最后24小时的两种处理后，DS淋巴细胞中染色单体型畸变也有显著增加。
