Andersson H C, Tranberg E A, Uggla A H, Zetterberg G
Mutat Res. 1980 Dec;73(2):387-401. doi: 10.1016/0027-5107(80)90203-1.
Workers in a Swedish factory making boats from plastics reinforced with glass fibre are exposed to a variety of chemicals, including styrene which is mutagenic after metabolic activation. The concentration of styrene in the air was measured in the breathing zones of workers occupied with various processes in boat making. Samples of air were taken 6 times during the years 1973-1978. The total exposure to styrene for the workers during this period was calculated and expressed as the average concentration in mg per m3 air during an 8-h workshift multiplied by the number of years of employment. A low-dose group (mean = 137 mg x m-3) and a high-dose group)mean - 1204 mg x m-3) were identified. Blood samples were taken in 1978 from workers belonging to the exposed groups and from a matched control group of employees in the same factory not exposed to styrene. Lymphocytes were cultured and examined for chromosomal aberrations and sister-chromatid exchanges. Exposed workers had a significantly (p less than 0.001) higher number of chromosomal aberrations (36 persons, mean = 7.9 aberrations/100 cells) compared with employees in the control group (37 persons, mean = 3.2 aberrations/100 cells). There was no significant difference between the mean values of the number of chromosomal aberrations between the highly exposed and the less exposed groups. But in the less exposed group there was an increase in the frequency of chromosomal aberrations with increasing exposure to styrene (r = 0.576). In the highly exposed group this dose response was not observed (r = 0.231). For the frequency of sister-chromatid exchanges (SCE) a slight (p less than 0.05) increase was found in the styrene-exposed group (20 persons, mean = 8.4 SCE/cell). The control group (21 persons) had a mean value of 7.5 SCE/cell. Again there was no difference between the highly exposed and the less exposed groups. Other environmental factors that may have clastogenic effects were studied, but multiple regression analysis failed to show a candidate responsible for the increase in chromosomal abnormalities in the exposed group.
瑞典一家用玻璃纤维增强塑料制造船只的工厂里，工人们接触到各种化学物质，其中包括苯乙烯，苯乙烯经代谢活化后具有致突变性。在造船过程中，对从事不同工序的工人呼吸区域的空气中苯乙烯浓度进行了测量。1973年至1978年期间，共采集了6次空气样本。计算了这一时期工人接触苯乙烯的总量，并表示为8小时工作日期间每立方米空气中苯乙烯的平均浓度（毫克）乘以就业年限。确定了一个低剂量组（平均值 = 137毫克·立方米⁻³）和一个高剂量组（平均值 = 1204毫克·立方米⁻³）。1978年从暴露组工人以及同一工厂未接触苯乙烯的匹配对照组员工中采集了血样。培养淋巴细胞并检查染色体畸变和姐妹染色单体交换情况。与对照组员工（37人，平均值 = 3.2个畸变/100个细胞）相比，暴露组工人的染色体畸变数量显著更高（36人，平均值 = 7.9个畸变/100个细胞）（p < 0.001）。高暴露组和低暴露组之间染色体畸变数量的平均值没有显著差异。但在低暴露组中，随着苯乙烯暴露量增加，染色体畸变频率上升（r = 0.576）。在高暴露组中未观察到这种剂量反应（r = 0.231）。对于姐妹染色单体交换（SCE）频率，在苯乙烯暴露组（20人，平均值 = 8.4次SCE/细胞）中发现有轻微增加（p < 0.05）。对照组（21人）的平均值为7.5次SCE/细胞。同样，高暴露组和低暴露组之间没有差异。研究了其他可能具有致断裂作用的环境因素，但多元回归分析未能找出导致暴露组染色体异常增加的因素。
