Huang Hui, Ba Yue, Cui Liuxin, Cheng Xuemin, Zhu Jingyuan, Zhang Yuling, Yan Pingping, Zhu Cairong, Kilfoy Briseis, Zhang Yawei
Department of Environmental Health, Zhengzhou University School of Public Health, Zhengzhou, China.
Community Dent Oral Epidemiol. 2008 Dec;36(6):517-22. doi: 10.1111/j.1600-0528.2007.00424.x. Epub 2008 Feb 18.
To investigate the relationship between dental fluorosis, polymorphisms in the COL1A2 gene, and serum calciotropic hormone levels.
We conducted a case-control study among children between 8 and 12 years of age with (n = 75) and without (n = 165) dental fluorosis in two counties in Henan Province, China. The PvuII and RsaI polymorphisms in the COL1A2 gene were genotyped using the PCR-RFLP procedure. Calcitonin and osteocalcin levels in the serum were measured using radioimmunassays.
Children carrying the homozygous genotype PP of COL1A2 PvuII had a significantly increased risk of dental fluorosis (OR =4.85, 95% CI: 1.22-19.32) compared to children carrying the homozygous genotype pp in an endemic fluorosis village (EFV). However, the risk (OR = 1.07, 95% CI: 0.45-2.52) was not elevated when the control population was recruited from a non-endemic fluorosis village. Additionally, fluoride levels in urine and osteocalcin levels in serum were found to be significantly lower in controls from non-endemic villages compared to cases. However, the differences in fluoride and osteocalcin levels were not observed when cases were compared to a control population from endemic fluorosis villages.
This study provides the first evidence of an association between polymorphisms in the COL1A2 gene with dental fluorosis in high fluoride exposed populations. Future studies are needed to confirm the association.
研究氟斑牙、COL1A2基因多态性与血清钙调节激素水平之间的关系。
我们在中国河南省两个县对8至12岁患氟斑牙（n = 75）和未患氟斑牙（n = 165）的儿童进行了一项病例对照研究。采用PCR-RFLP方法对COL1A2基因中的PvuII和RsaI多态性进行基因分型。使用放射免疫分析法测定血清中的降钙素和骨钙素水平。
与地方性氟中毒村（EFV）中携带纯合子基因型pp的儿童相比，携带COL1A2 PvuII纯合子基因型PP的儿童患氟斑牙的风险显著增加（OR = 4.85，95%CI：1.22 - 19.32）。然而，当对照人群从非地方性氟中毒村招募时，风险（OR = 1.07，95%CI：0.45 - 2.52）并未升高。此外，发现非地方性村庄的对照者尿氟水平和血清骨钙素水平显著低于病例组。然而，将病例与地方性氟中毒村的对照人群进行比较时，未观察到氟和骨钙素水平的差异。
本研究首次提供了COL1A2基因多态性与高氟暴露人群氟斑牙之间存在关联的证据。需要进一步的研究来证实这种关联。
