Ba Yue, Li Hai-Xia, Yin Gong-Ju, Wu Wei-Hua, Yu Bo, Cheng Xue-Min, Cui Liu-Xin
Department of Environmental Health, Zhengzhou University School of Public Health, Zhengzhou, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2009 Sep;40(5):869-72.
To explore the distribution of ER Rsa I genotype in children who lived in the areas with or without high fluoride, and evaluate the relationship between ER Rsa I gene polymorphism and children's dental fluorosis.
Children aged 8 to 12 years, born and raised in high fluoride areas and control areas in two counties of Henan Province were recruited. The Rsa I marker of ER gene was genotyped in 237 children composed of both dental fluorosis cases and controls by PCR-RFLP procedure. Urine fluoride was detected with fluoride ion selective electrode method.
The frequency distribution of ER Rsa I genotype was rr 60.81% (45/74), Rr 27.02% (20/74), RR 12.16% (9/74) in children with fluorosis; rr 73.91% (51/69), Rr 20.29% (14/69), RR 5.80% (4/69) in children without fluorosis from high fluoride areas, and rr 63.83% (60/94), Rr 34.04% (32/94), RR 2.13% (2/94) in the children without fluorosis from control areas respectively. There were no significant differences in the three groups (P>0.05), but children carrying allele R of ER Rsa I had a significantly increased risk of dental fluorosis (OR=1.821, 95% CI: 1.013-3.274) compared to children carrying the allele r in endemic fluorosis areas.
Although no significant difference was found in ER Rsa I genotype between cases and non-dental fluorosis in endemic fluorosis areas, children carrying R allele of ER Rsa I had a higher risk compared to children carrying r allele, and the further study is needed.
探讨居住在高氟和非高氟地区儿童雌激素受体（ER）Rsa I基因型的分布情况，并评估ER Rsa I基因多态性与儿童氟斑牙之间的关系。
选取河南省两个县高氟地区和对照地区出生并长大的8至12岁儿童。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法，对237例既有氟斑牙病例又有对照的儿童进行ER基因的Rsa I标记基因分型。用氟离子选择电极法检测尿氟。
氟斑牙儿童中ER Rsa I基因型频率分布为rr 60.81%（45/74）、Rr 27.02%（20/74）、RR 12.16%（9/74）；高氟地区非氟斑牙儿童中rr 73.91%（51/69）、Rr 20.29%（14/69）、RR 5.80%（4/69）；对照地区非氟斑牙儿童中rr 63.83%（60/94）、Rr 34.04%（32/94）、RR 2.13%（2/94）。三组间差异无统计学意义（P>0.05），但在地方性氟中毒地区，携带ER Rsa I等位基因R的儿童患氟斑牙的风险显著增加（OR=1.821，95%可信区间：1.013 - 3.274），与携带等位基因r的儿童相比。
虽然在地方性氟中毒地区氟斑牙病例与非氟斑牙儿童的ER Rsa I基因型未发现显著差异，但携带ER Rsa I等位基因R的儿童比携带r等位基因的儿童风险更高，需要进一步研究。
