Maragos C M, Appell M, Lippolis V, Visconti A, Catucci L, Pascale M
Mycotoxin Research Unit, USDA-ARS-NCAUR, Peoria, IL 61604, USA.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2008 Feb;25(2):164-71. doi: 10.1080/02652030701564555.
Cyclodextrins, cyclic oligosaccharides composed of amylose subunits, are known to interact with mycotoxins. The interactions may be useful to analytical chemists by altering the properties of the mycotoxin of interest, namely the chromatographic properties, electrophoretic properties, fluorescence, or absorption of these fungal metabolites. Practical applications of these effects have been the incorporation of cyclodextrins into high-performance liquid chromatography and capillary electrophoresis methods for mycotoxin detection. Specific mycotoxins include those with a native fluorescence such as the aflatoxins, ochratoxin A (OTA) and zearalenone (ZEN) as well as those that can be rendered fluorescent through derivatization, such as T-2 toxin. The literature describing the applications of cyclodextrins in mycotoxin analysis is reviewed and an attempt to extend the use of cyclodextrins to the detection of labelled T-2 toxin is presented. Twenty cyclodextrins were evaluated for their ability to enhance the fluorescence emission of T-2 toxin derivatized with pyrene-1-carbonyl cyanide (T2-Pyr). This evaluation revealed that heptakis (2,6-di-O-methyl)-beta-cyclodextrin (DIMEB), in particular, enhanced T2-Pyr fluorescence. DIMEB was used as a buffer modifier in a capillary electrophoresis-laser-induced fluorescence (CE-LIF) method for detecting T-2 in maize. Because of the effects that certain cyclodextrins have, especially under aqueous conditions, they may make useful additives for a variety of mycotoxin analytical methods.
环糊精是由直链淀粉亚基组成的环状寡糖，已知其能与霉菌毒素相互作用。这些相互作用对于分析化学家可能是有用的，因为它们可以改变目标霉菌毒素的性质，即这些真菌代谢产物的色谱性质、电泳性质、荧光或吸收特性。这些效应的实际应用包括将环糊精纳入用于霉菌毒素检测的高效液相色谱法和毛细管电泳法中。特定的霉菌毒素包括那些具有天然荧光的毒素，如黄曲霉毒素、赭曲霉毒素A（OTA）和玉米赤霉烯酮（ZEN），以及那些可以通过衍生化变为荧光性的毒素，如T-2毒素。本文综述了描述环糊精在霉菌毒素分析中应用的文献，并尝试将环糊精的使用扩展到标记T-2毒素的检测。评估了20种环糊精增强芘-1-羰基氰化物衍生化的T-2毒素（T2-Pyr）荧光发射的能力。该评估表明，特别是七（2,6-二-O-甲基）-β-环糊精（DIMEB）增强了T2-Pyr的荧光。DIMEB被用作毛细管电泳-激光诱导荧光（CE-LIF）法检测玉米中T-2毒素的缓冲液改性剂。由于某些环糊精所具有的效应，特别是在水性条件下，它们可能成为各种霉菌毒素分析方法的有用添加剂。
