Emmersen Jeppe
Department of Biochemistry, Chemistry and Environmental Engineering, University of Aalborg, Aalborg, Denmark.
Methods Mol Biol. 2008;387:143-50. doi: 10.1007/978-1-59745-454-4_11.
The Long serial analysis of gene expression (SAGE) protocol generates ditags from tags with overlapping overhangs, thereby increasing the probability of duplicate ditag formation in LongSAGE. In this chapter, a tool is presented that facilitates the analysis of duplicate ditags in LongSAGE studies to determine whether they should be included or not.