使用深度敏感光纤探头对口腔上皮组织进行自体荧光和漫反射光谱分析。
Autofluorescence and diffuse reflectance spectroscopy of oral epithelial tissue using a depth-sensitive fiber-optic probe.
作者信息
Schwarz Richard A, Gao Wen, Daye Dania, Williams Michelle D, Richards-Kortum Rebecca, Gillenwater Ann M
机构信息
Department of Bioengineering MS 142, Rice University, 6100 Main Street, Keck Hall Suite 116, Houston, Texas 77005, USA.
出版信息
Appl Opt. 2008 Feb 20;47(6):825-34. doi: 10.1364/ao.47.000825.
Abstract
Optical spectroscopy can provide useful diagnostic information about the morphological and biochemical changes related to the progression of precancer in epithelial tissue. As precancerous lesions develop, the optical properties of both the superficial epithelium and underlying stroma are altered; measuring spectral data as a function of depth has the potential to improve diagnostic performance. We describe a clinical spectroscopy system with a depth-sensitive, ball lens coupled fiber-optic probe for noninvasive in vivo measurement of oral autofluorescence and diffuse reflectance spectra. We report results of spectroscopic measurements from oral sites in normal volunteers and in patients with neoplastic lesions of the oral mucosa; results indicate that the addition of depth selectivity can enhance the detection of optical changes associated with precancer.
摘要
光学光谱学能够提供与上皮组织癌前病变进展相关的形态学和生化变化的有用诊断信息。随着癌前病变的发展，表层上皮和下层基质的光学特性都会发生改变；测量作为深度函数的光谱数据有可能提高诊断性能。我们描述了一种临床光谱学系统，该系统配备了深度敏感的球透镜耦合光纤探头，用于无创体内测量口腔自体荧光和漫反射光谱。我们报告了正常志愿者和口腔黏膜肿瘤性病变患者口腔部位的光谱测量结果；结果表明，增加深度选择性可以增强对与癌前病变相关的光学变化的检测。
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