Sabasiñska Anna, Zoch-Zwierz Walentyna, Wasilewska Anna, Porowski Tadeusz
1st Department of Pediatrics, Medical University of Białystok, ul. Waszyngtona 17, Białystok, Poland.
Pediatr Nephrol. 2008 May;23(5):769-74. doi: 10.1007/s00467-007-0723-8. Epub 2008 Feb 21.
High-grade vesicoureteric reflux (VUR) promotes the development of renal nephropathy (RN) due to scar formation. This process involves transforming growth factor beta-1 (TGF beta(1)), which stimulates production of the extracellular matrix proteins, including laminin (LN). The aim of the study was to assess LN and TGF beta(1) concentration according to VUR grade. The study group (1) consisted of 54 patients aged 6.23 +/- 4.15 years with VUR, including: A, 19 with grade II; B, 19 with grade III; and C, 16 with grades IV or V reflux. The control group (2) contained 27 healthy patients aged 6.76 +/- 4.02 years. LN and total TGF beta(1) concentrations in serum and urine were determined by the immunoenzymatic (EIA) method. To assess total serum TGF beta(1) levels, we used a solid-phase enzyme-linked immunosorbent assay (ELISA). Both serum and urinary levels of LN and TGF beta(1) in VUR patients were higher compared with controls (p < 0.05). The highest urinary concentration of LN and TGF beta(1) was found in subgroup C. A positive correlation was noted between urinary TGF beta(1) and LN. Increased TGF-beta(1) and LN levels in urine of high-grade VUR children suggests a potential role in fibrogenesis. Further trials are needed to investigate the role of serum and urinary LN level in VUR children.
重度膀胱输尿管反流（VUR）会因瘢痕形成而促进肾肾病（RN）的发展。这一过程涉及转化生长因子β-1（TGFβ(1)），它会刺激包括层粘连蛋白（LN）在内的细胞外基质蛋白的产生。本研究的目的是根据VUR分级评估LN和TGFβ(1)的浓度。研究组（1）由54例年龄为6.23±4.15岁的VUR患者组成，包括：A组，19例II级患者；B组，19例III级患者；C组，16例IV级或V级反流患者。对照组（2）包含27例年龄为6.76±4.02岁的健康患者。血清和尿液中的LN及总TGFβ(1)浓度采用免疫酶法（EIA）测定。为评估血清总TGFβ(1)水平，我们使用了固相酶联免疫吸附测定法（ELISA）。VUR患者的血清和尿液中LN及TGFβ(1)水平均高于对照组（p<0.05）。C亚组中LN和TGFβ(1)的尿浓度最高。尿中TGFβ(1)与LN之间存在正相关。重度VUR儿童尿液中TGF-β(1)和LN水平升高表明其在纤维生成中可能发挥作用。需要进一步试验来研究血清和尿液LN水平在VUR儿童中的作用。
