Galien R, Mercier G, Garcette M, Emanoil-Ravier R
Laboratoire des Retrovirus et Retrotransposons des Vertébrés, UPR 0043 CNRS, Hopital Saint-Louis, Paris, France.
Oncogene. 1991 May;6(5):849-55.
To understand the mechanism responsible for the high expression of Intracisternal A Particles (IAP) in murine cells transformed by the Kirsten Mouse Sarcoma Virus (Ki-MSV), we have investigated the effect of p21ras on IAP transcription. By transient cotransfections of IAP LTR-CAT plasmids with v-Ki-ras and c-Ki-ras expression vectors, we have found that p21v-Ki-ras, and the p21c-Ki-ras to a lesser extent, stimulate the promoter activity of the 5' IAP LTR. We constructed several plasmids containing the CAT gene under control of IAP LTRs deleted in different regions. CAT assays demonstrate that the ras responsive sequence is a 16 bp oligonucleotide containing an effective c-AMP Response Element (CRE) TGACGTCA, which is located in the U3 region, 20 bp upstream of the CAAT box.
为了解在柯斯顿小鼠肉瘤病毒（Ki-MSV）转化的鼠细胞中胞内A颗粒（IAP）高表达的机制，我们研究了p21ras对IAP转录的影响。通过将IAP LTR-CAT质粒与v-Ki-ras和c-Ki-ras表达载体进行瞬时共转染，我们发现p21v-Ki-ras以及程度较轻的p21c-Ki-ras能刺激5'IAP LTR的启动子活性。我们构建了几个含有在不同区域缺失的IAP LTR控制下的CAT基因的质粒。CAT分析表明，ras反应序列是一个16bp的寡核苷酸，包含一个有效的c-AMP反应元件（CRE）TGACGTCA，它位于U3区域，CAAT框上游20bp处。
