Chen Ying, Xu Liangjun, Lin Jinming, Chen Guonan
Ministry of Education Key Laboratory of Analysis and Detection Technology for Food Safety, Fuzhou University, Fuzhou, Fujian, PR China.
Electrophoresis. 2008 Mar;29(6):1302-7. doi: 10.1002/elps.200700594.
A CE with LIF detection was developed for separation and determination of bradykinin (BK)-related peptides, such as BK, kallidin (Kal), and neurokinin A (NKA). BK-related peptides were derivatized with FITC prior to CE-LIF analysis. Sodium borate 10 mmol/L at pH 9.5 was selected as derivatization media in order to get the high efficiency. Three peptides were baseline-separated within 10 min by using 110 mmol/L sodium borate-sodium hydroxide solution at pH 10.0 as the running buffer. Concentration detection limits (S/N = 3) for BK, Kal, and NKA were 0.08, 0.5, and 0.2 nmol/L, respectively. Meanwhile we have also developed a simple, quick, and sensitive large-volume sample stacking (LVSS) technique for CE-LIF detection of BK, Kal, and NKA. By using this stacking technique, the detection limits (S/N = 3) for BK, Kal, and NKA were 0.02, 0.05, and 0.04 nmol/L, respectively. This method has been applied to the assay of human saliva and cerebrospinal fluid with satisfactory results.
开发了一种用于分离和测定缓激肽（BK）相关肽（如BK、胰激肽（Kal）和神经激肽A（NKA））的毛细管电泳-激光诱导荧光检测（CE-LIF）方法。在进行CE-LIF分析之前，BK相关肽用异硫氰酸荧光素（FITC）进行衍生化。选择pH 9.5的10 mmol/L硼酸钠作为衍生化介质以获得高效衍生化效果。以pH 10.0的110 mmol/L硼酸钠-氢氧化钠溶液作为运行缓冲液，三种肽在10分钟内实现了基线分离。BK、Kal和NKA的浓度检测限（信噪比S/N = 3）分别为0.08、0.5和0.2 nmol/L。同时，我们还开发了一种简单、快速且灵敏的大体积样品堆积（LVSS）技术用于CE-LIF检测BK、Kal和NKA。通过使用这种堆积技术，BK、Kal和NKA的检测限（S/N = 3）分别为0.02、0.05和0.0４nmol/L。该方法已应用于人体唾液和脑脊液的检测，结果令人满意。
