Bi Weiwen, Lei Shaorong, Yang Xiupei, Xu Zemin, Yuan Hongyan, Xiao Dan, Choi Martin M F
College of Chemistry, Sichuan University, Chengdu 610065, PR China.
Talanta. 2009 May 15;78(3):1167-72. doi: 10.1016/j.talanta.2009.01.042. Epub 2009 Jan 30.
Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n=7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of D- and L-Tyr derivatives were 2.9 and 2.2 micromol/L (S/N=3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution.
已开发出毛细管电泳（CE）与光纤发光二极管诱导荧光检测联用技术用于酪氨酸（Tyr）对映体的分离。R(-)-4-(3-异硫氰酸吡咯烷-1-基)-7-(N,N-二甲基氨基磺酰基)-2,1,3-苯并恶二唑用作手性荧光标记试剂对Tyr进行衍生化。研究了pH值、运行缓冲液浓度和施加电压对 enantioselectivity 的影响。最佳CE条件为15 mmol/L硼酸盐运行缓冲液（pH 10.5）和14 kV施加电压。迁移时间和峰面积的变异系数（n = 7）分别小于0.2%和2.0%，获得了良好的重现性。D-和L-Tyr衍生物的检测限分别为2.9和2.2 μmol/L（S/N = 3）。该方法已成功应用于市售氨基酸口服溶液中Tyr的测定。
