Davis Barry D, Brodbelt Jennifer S
Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, TX 78712, USA.
J Mass Spectrom. 2008 Aug;43(8):1045-52. doi: 10.1002/jms.1381.
The trend in the extent of homolytic saccharide cleavage is reported for a series of deprotonated flavonol 3-O-glycosides upon collision-induced dissociation (CID) in a quadrupole ion trap mass spectrometer. The second-generation product ions from the primary Y(0) and Y(0)- H product ions were also identified. It was determined that the structure of both the aglycon and the saccharide portions of the flavonoid glycoside are pivotal in inducing radical cleavage. In contrast to earlier work on this subject reported for a smaller group of flavonols, the correlation between the degree of B-ring hydroxylation and the extent of radical saccharide cleavage showed several notable exceptions in the present work. Homolytic cleavage was also investigated in the context of using tandem mass spectrometry to identify the aglycon portions of flavonoid glycosides.
报道了在四极杆离子阱质谱仪中，一系列去质子化黄酮醇3 - O -糖苷在碰撞诱导解离（CID）时，均裂糖裂解程度的趋势。还鉴定了来自初级[Y(0)]⁻和[Y(0) - H]⁻产物离子的第二代产物离子。已确定黄酮糖苷的苷元结构和糖部分在诱导自由基裂解中都起着关键作用。与早期针对较小一组黄酮醇的该主题研究不同，在本研究中，B环羟基化程度与自由基糖裂解程度之间的相关性出现了几个显著例外。还在使用串联质谱法鉴定黄酮糖苷苷元部分的背景下研究了均裂裂解。
