Collins T M, Caimi R, Lynch P R, Sheffield J, Mitra A, Stueber K, Smith Y R
Department of Surgery, Temple University School of Medicine, Philadelphia, PA.
Scand J Plast Reconstr Surg Hand Surg. 1991;25(1):5-7. doi: 10.3109/02844319109034915.
Hyperbaric oxygen has been established as an acceptable treatment for the chronic healing wound. Nicotinamide has been shown to be angiogenic and accelerate the physiologic process following wounding. Therefore both nicotinamide and hyperbaric oxygen were evaluated to enhance flap survival in an island pedicle skin flap model. These two treatment modalities were evaluated alone and in combination to assess if there is an addictive effect to enhance flap survival. Forty Sprague-Dawley male rats (weight 300-350 grams) were treated for 14 days preoperatively 1 day post-operatively with either 400 mg of nicotinamide i.p. or saline i.p. On day 14, a 7 X 7 cm island pedicle skin flap was elevated ligating the left inferior epigastric neurovascular pedicle and were sutured in their normal position. Twenty animals then underwent hyperbaric oxygen treatments. Forty-eight hours post-operatively animals were re-anesthetized and were given a single injection of fluorescein (25 mg/kg) via the tail vein. The % survival of the flap and SEM of the groups are as follows: Saline 45.67 +/- 31.14, nicotinamide 85.30 +/- 9.24, saline-hyperbaric oxygen 76.70 +/- 9.42 and nicotinamide-hyperbaric oxygen 90.86 +/- 3.94 with statistical significance of p less than 0.01. Nicotinamide appears to be another acceptable therapeutic modality in the management of the acceleration of wound healing.
高压氧已被确立为慢性愈合伤口的一种可接受的治疗方法。烟酰胺已被证明具有促血管生成作用，并能加速伤口愈合后的生理过程。因此，在岛状蒂皮瓣模型中评估了烟酰胺和高压氧对提高皮瓣存活率的作用。单独及联合评估这两种治疗方式，以确定是否存在提高皮瓣存活率的相加效应。40只体重300 - 350克的雄性Sprague-Dawley大鼠，术前14天及术后1天，腹腔注射400毫克烟酰胺或生理盐水。在第14天，掀起一个7×7厘米的岛状蒂皮瓣，结扎左下腹壁神经血管蒂，并缝合于正常位置。然后对20只动物进行高压氧治疗。术后48小时，动物再次麻醉，经尾静脉单次注射荧光素（25毫克/千克）。各实验组皮瓣存活率及标准误如下：生理盐水组45.67±31.14，烟酰胺组85.30±9.24，生理盐水-高压氧组76.70±9.42，烟酰胺-高压氧组90.86±3.94，p值小于0.01，具有统计学意义。烟酰胺似乎是促进伤口愈合的另一种可接受的治疗方式。
