Prada Flavia S, Arrunategui Gino, Alves Marcos C, Ferreira Marcos C, Zumiotti Arnaldo V
Section of Reconstructive Microsurgery, Division of Traumatology and Orthopaedic Surgery, Hospital das Clínicas, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil.
Microsurgery. 2002;22(8):352-60. doi: 10.1002/micr.10073.
The effect of allopurinol, superoxide-dismutase, and hyperbaric oxygen was compared on axial pattern skin flap survival. An abdominal flap based on the inferior epigastric pedicle was raised in rats for this purpose. Three experimental groups were studied. In the first group, adult male Wistar rats received 50 mg/kg I.P. of allopurinol. The second received 20,000 I.U./kg of superoxide-dismutase. The third group was submitted to hyperbaric oxygen therapy. The flaps were exposed to 8-h warm ischemia. Flap survival was evaluated on postoperative day 7. All flaps survived, and the mean survival areas were 63.53%, 83.03%, and 55.98%, respectively, in the allopurinol, superoxide-dismutase, and hyperbaric groups. The percentage of flap necrosis was significantly smaller in all experimental groups when compared to controls (P < 0.05). It was clear that the superoxide-dismutase group had better results on axial pattern ischemic skin flap survival, under the tested conditions. The tested methods improved flap survival to ischemic injury, and the flap designed is a reliable model for further investigations.
比较了别嘌呤醇、超氧化物歧化酶和高压氧对轴型皮瓣存活的影响。为此在大鼠身上掀起了以腹壁下血管蒂为基础的腹部皮瓣。研究了三个实验组。第一组成年雄性Wistar大鼠腹腔注射50mg/kg别嘌呤醇。第二组注射20000IU/kg超氧化物歧化酶。第三组接受高压氧治疗。皮瓣经历8小时的热缺血。在术后第7天评估皮瓣存活情况。所有皮瓣均存活，别嘌呤醇组、超氧化物歧化酶组和高压氧组的平均存活面积分别为63.53%、83.03%和55.98%。与对照组相比，所有实验组皮瓣坏死的百分比均显著降低（P<0.05）。显然，在测试条件下，超氧化物歧化酶组在轴型缺血皮瓣存活方面有更好的结果。所测试的方法提高了皮瓣对缺血损伤的存活能力，并且所设计的皮瓣是用于进一步研究的可靠模型。
