Gaspar L, Fernandez-Durango R, Seidah N G, Chrétien M, Gutkowska J
J. A. DeSève Laboratory of Molecular, Neuroendocrinology, Clinical Research Institute of Montreal, Quebec, Canada.
Endocrinology. 1991 Jul;129(1):559-61. doi: 10.1210/endo-129-1-559.
Specific proANF mRNA was demonstrated by the polymerase chain reaction (PCR) technique in ciliary body and chorioidea tissue extracts but not in the retina of the rat eye. However, immunoreactive (IR) ANF was detected in all of these tissues by a specific and sensitive radioimmunoassay (RIA). Since ANF seems to be involved in the maintenance of intraocular pressure, the regulation of ANF gene expression in these distinct eye tissues could be an important factor in the pathogenesis of glaucoma.
通过聚合酶链反应（PCR）技术在大鼠眼的睫状体和脉络膜组织提取物中检测到特异性的前心钠素（proANF）mRNA，但在视网膜中未检测到。然而，通过特异性灵敏放射免疫测定法（RIA）在所有这些组织中均检测到免疫反应性（IR）心钠素（ANF）。由于心钠素似乎参与眼内压的维持，因此这些不同眼组织中心钠素基因表达的调节可能是青光眼发病机制中的一个重要因素。
