[兔脉络膜、视网膜及睫状体中存在心房利钠肽]

[Existence of atrial natriuretic peptide in choroid, retina and ciliary body in rabbits].

作者信息

García de Lacoba M, Fernández-Durango R, Triviño A, Ramírez J M, Ramírez A I, Salazar J J, Fernández-Cruz A, Gutkowska J

机构信息

Centro de Diabetes e Hipertensión, Departamento de Medicina Interna III, Hospital Universitario San Carlos, Madrid, España.

出版信息

Rev Esp Fisiol. 1991 Jun;47(2):91-6.

PMID:1833802

Abstract

The presence of atrial natriuretic factor (ANF) in choroid, retina and ciliary body of rabbit eyes has been studied. The peptide was extracted by reverse phase chromatography using Sep-Pak C18 cartridges. Immunoreactive ANF (Ir-ANF) was measured by a specific RIA. In the choroid, retina and ciliary body the ir-ANF concentrations were 444 +/- 94, 67 +/- 12 and 223 +/- 75 pg/g tissue weight (n = 16), respectively. The biological activity of the acid extract ANF was evaluated by a 125I-ANF binding assay to a retinal particulate preparation. Results demonstrate the existence of biological active ANF in choroid, retina and ciliary body of rabbit eyes. The presence of ANF and its specific receptors in the ocular tissues suggests that this hormone might play a physiological role in the intraocular fluid homeostasis.

摘要

对兔眼脉络膜、视网膜和睫状体中的心钠素（ANF）进行了研究。使用Sep-Pak C18柱通过反相色谱法提取该肽。通过特异性放射免疫分析法（RIA）测定免疫反应性心钠素（Ir-ANF）。在脉络膜、视网膜和睫状体中，Ir-ANF浓度分别为444±94、67±12和223±75 pg/g组织重量（n = 16）。通过125I-ANF与视网膜微粒体制剂的结合试验评估酸性提取物心钠素的生物活性。结果表明兔眼脉络膜、视网膜和睫状体中存在生物活性心钠素。眼组织中的心钠素及其特异性受体表明该激素可能在眼内液稳态中发挥生理作用。