Elrayah I E, Rhaman M A, Karamalla L T, Khalil K M, Büscher P
Trypanosomiasis Unit, Tropical Medicine Research Institute, Khartoum, Sudan.
East Mediterr Health J. 2007 Sep-Oct;13(5):1098-107. doi: 10.26719/2007.13.5.1098.
A survey was conducted in a low-endemic and in a non-endemic area of Sudan to evaluate the specificity and efficiency of different serological antibody detection techniques for Trypanosoma brucei gambiense. Comparisons were made of the card agglutination test for trypanosomiasis (CATT) on diluted blood, on diluted plasma and on eluates from blood dried on filter paper, the LATEX test on diluted plasma and an ELISA on diluted plasma and filter paper. The specificities of all the serological tests were not significantly different from CATT on diluted blood (99.5%). The specificity of CATT on diluted blood was similar (99.3%). The highest sensitivities (100%) were observed with CATT on diluted blood and with CATT and LATEX on diluted plasma. CATT on diluted blood was more cost-efficient than the classic test, CATT on whole blood.
在苏丹的一个低流行区和一个非流行区进行了一项调查，以评估不同血清学抗体检测技术对布氏冈比亚锥虫的特异性和效率。对稀释血液、稀释血浆以及滤纸干燥血洗脱液上的锥虫病卡片凝集试验（CATT）、稀释血浆上的乳胶试验以及稀释血浆和滤纸上的酶联免疫吸附测定（ELISA）进行了比较。所有血清学检测的特异性与稀释血液上的CATT（99.5%）无显著差异。稀释血液上的CATT特异性相似（99.3%）。在稀释血液上的CATT、稀释血浆上的CATT和乳胶试验中观察到最高灵敏度（100%）。稀释血液上的CATT比经典的全血CATT更具成本效益。
