Ow Saw Yen, Cardona Tanai, Taton Arnaud, Magnuson Ann, Lindblad Peter, Stensjö Karin, Wright Phillip C
Biological & Environmental Systems Group, Department of Chemical and Process Engineering, The University of Sheffield, Sheffield S13JD, UK.
J Proteome Res. 2008 Apr;7(4):1615-28. doi: 10.1021/pr700604v. Epub 2008 Feb 22.
The filamentous cyanobacterium Nostoc sp. strain PCC 7120 is capable of fixing atmospheric nitrogen. The labile nature of the core process requires the terminal differentiation of vegetative cells to form heterocysts, specialized cells with altered cellular and metabolic infrastructure to mediate the N2-fixing process. We present an investigation targeting the cellular proteomic expression of the heterocysts compared to vegetative cells of a population cultured under N2-fixing conditions. New 8-plex iTRAQ reagents were used on enriched replicate heterocyst and vegetative cells, and replicate N2-fixing and non-N2-fixing filaments to achieve accurate measurements. With this approach, we successfully identified 506 proteins, where 402 had confident quantifications. Observations provided by purified heterocyst analysis enabled the elucidation of the dominant metabolic processes between the respective cell types, while emphasis on the filaments enabled an overall comparison. The level of analysis provided by this investigation presents various tools and knowledge that are important for future development of cyanobacterial biohydrogen production.
丝状蓝细菌集胞藻属PCC 7120菌株能够固定大气中的氮。核心过程的不稳定性要求营养细胞进行终末分化以形成异形胞，即具有改变的细胞和代谢基础设施的特殊细胞，以介导固氮过程。我们进行了一项研究，旨在比较在固氮条件下培养的群体中异形胞与营养细胞的细胞蛋白质组表达。新型8重iTRAQ试剂用于富集的重复异形胞和营养细胞，以及重复的固氮和非固氮丝状体，以实现准确测量。通过这种方法，我们成功鉴定了506种蛋白质，其中402种有可靠的定量结果。纯化异形胞分析提供的观察结果有助于阐明各细胞类型之间的主要代谢过程，而对丝状体的关注则有助于进行整体比较。这项研究所提供的分析水平展示了各种对蓝藻生物制氢未来发展很重要的工具和知识。
